Ostrowski W, Bhargava A K, Dziembor E, Gizler M, Gryszkiewicz J, Barnard E A
Biochim Biophys Acta. 1976 Nov 26;453(1):262-9. doi: 10.1016/0005-2795(76)90272-5.
Human prostatic acid phosphatase I, a glycoprotein, has been analyzed with respect to its quantiative carbohydrate composition and its fluorescence, optical rotatory and circular dichroic spectra. The protein of 89 000 molecular weight has 38 to 41 carbohydrate residues attached, of which 3 residues are fucose, 4 are galactose, 11 mannose, 15 glucosamine and there are 7 to 8 residues of sialic acid. The native glycoprotein contains about 30% alpha-helix as estimated from the rotational and dichroic spectra. Upon removal of sialic acid by neuraminidase treatment, there is a small increase in this value, while the fluorescence intensity at he emission maximum (357 nm) is distinctly increased. These effects suggest that an interaction exists of the sialic acid group with parts of the native protein. Allowance is made for the contribution of the carbohydrate components in interpreting the spectra in structural terms.
人前列腺酸性磷酸酶I是一种糖蛋白，已对其定量碳水化合物组成以及荧光、旋光和圆二色光谱进行了分析。这种分子量为89000的蛋白质连接有38至41个碳水化合物残基，其中3个残基是岩藻糖，4个是半乳糖，11个是甘露糖，15个是葡糖胺，还有7至8个唾液酸残基。根据旋转光谱和二色光谱估计，天然糖蛋白含有约30%的α-螺旋。用神经氨酸酶处理去除唾液酸后，该值略有增加，而发射最大值（357nm）处的荧光强度明显增加。这些效应表明唾液酸基团与天然蛋白质的某些部分存在相互作用。在从结构角度解释光谱时考虑了碳水化合物成分的贡献。
