菜豆叶绿体转移核糖核酸与叶绿体脱氧核糖核酸的杂交

Hybridization of bean chloroplast transfer RNAs to chloroplast DNA.

作者信息

Steinmetz A, Well J H

出版信息

Biochim Biophys Acta. 1976 Dec 13;454(3):429-35. doi: 10.1016/0005-2787(76)90269-0.

DOI:10.1016/0005-2787(76)90269-0

Abstract

Bean (Phaseolus vulgaris) chloroplast tRNAsLeu and tRNAsPhe hybridize to chloroplast DNA, whereas the corresponding cytoplasmic tRNA species do not, suggesting that chloroplast transfer RNAs are coded for by chloroplast DNA. The hybridization of the three chloroplast tRNAsLeu or of the two tRNAsPhe isoacceptors is not additive, and the isoacceptors compete with each other in the hybridization to chloroplast DNA, suggesting that these isoacceptors are coded for by the same gene(s) and differ only in the extent of post-transcriptional modification. Although hererologous aminoacylation reactions and comparisons of base composition suggest a resemblance between chloroplast and procaryotic tRNAs, only a slight cross hybridization reaction was observed between chloroplast and Escherichia coli leucyl- or phenylalanyl-tRNAs and DNAs.

摘要

菜豆（Phaseolus vulgaris）叶绿体的tRNAsLeu和tRNAsPhe与叶绿体DNA杂交，而相应的细胞质tRNA种类则不杂交，这表明叶绿体转移RNA是由叶绿体DNA编码的。三种叶绿体tRNAsLeu或两种tRNAsPhe同工受体的杂交不是累加性的，并且同工受体在与叶绿体DNA的杂交中相互竞争，这表明这些同工受体由相同的基因编码，只是在转录后修饰程度上有所不同。尽管异源氨酰化反应和碱基组成的比较表明叶绿体和原核生物tRNA之间存在相似性，但在叶绿体与大肠杆菌亮氨酰 - 或苯丙氨酰 - tRNA及DNA之间仅观察到轻微的交叉杂交反应。

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