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玉米叶绿体 DNA 的转移 RNA 基因。

Transfer RNA genes ofZea mays chloroplast DNA.

机构信息

The Biological Laboratories, Harvard University, Cambridge, Massachusetts, USA.

出版信息

Plant Mol Biol. 1983 May;2(3):141-53. doi: 10.1007/BF01578514.

DOI:10.1007/BF01578514
PMID:24318208
Abstract

A minimum of 37 genes corresponding to tRNAs for 17 different amino acids have been localized on the restriction endonuclease cleavage site map of theZea mays chloroplast DNA molecule. Of these, 14 genes corresponding to tRNAs for 11 amino acids are located in the larger of the two single-copy regions which separate the two inverted copies of the repeat region. One tRNA gene is in the smaller single-copy region. Each copy of the large repeated sequence contains, in addition to the ribosomal RNA genes, 11 tRNA genes corresponding to tRNAs for 8 amino acids. The genes for tRNA2 (Ile) and tRNA(Ala) map in the ribosomal spacer sequence separating the 16S and 23S ribosomal RNA genes. The three isoaccepting species for the tRNAs(Leu) and the three for tRNAs(Ser), as well as the two isoaccepting species for tRNA(Asn), tRNA(Gly), tRNAs(Ile), tRNAs(Met), tRNAs(Thr), are shown to be encoded at different loci.Two independent methods have been used for the localization of tRNA genes on the physical map of the maize chloroplast DNA molecule: (a) cloned chloroplast DNA fragments were hybridized with radioactively-labelled total 4S RNAs, the hybridized RNAs were then eluted, and identified by two-dimensional polyacrylamide gel electrophoresis, and (b) individual tRNAs were(32)P-labelledin vitro and hybridized to DNA fragments generated by digestion of maize chloroplast DNA with various restriction endonucleases.

摘要

在玉米叶绿体 DNA 分子的限制内切酶酶切位点图谱上,已定位了至少 37 个基因,它们分别对应 17 种不同氨基酸的 tRNA。在这些基因中,14 个对应 11 种氨基酸的 tRNA 基因位于两个单拷贝区中的较大一个区内,这两个单拷贝区把重复区的两个反向拷贝分开。一个 tRNA 基因位于较小的单拷贝区内。除核糖体 RNA 基因外,每个重复序列的拷贝还含有 11 个对应 8 种氨基酸的 tRNA 基因。tRNA2(Ile)和 tRNA(Ala)基因位于分隔 16S 和 23S 核糖体 RNA 基因的核糖体间隔序列中。tRNAs(Leu)和 tRNAs(Ser)的三个同工接受体,以及 tRNA(Asn)、tRNA(Gly)、tRNAs(Ile)、tRNAs(Met)、tRNAs(Thr)的两个同工接受体,显示出在不同的基因座上被编码。两种独立的方法已被用于在玉米叶绿体 DNA 分子的物理图谱上定位 tRNA 基因:(a)用放射性标记的总 4S RNA 杂交克隆的叶绿体 DNA 片段,然后洗脱杂交的 RNA,并通过二维聚丙烯酰胺凝胶电泳进行鉴定,(b)体外标记个别 tRNA,并用各种限制内切酶消化玉米叶绿体 DNA 生成的 DNA 片段进行杂交。

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1
Transfer RNA genes ofZea mays chloroplast DNA.玉米叶绿体 DNA 的转移 RNA 基因。
Plant Mol Biol. 1983 May;2(3):141-53. doi: 10.1007/BF01578514.
2
Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA.菠菜叶绿体转移RNA的分级分离与鉴定及其基因在叶绿体DNA限制酶切图谱上的定位
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3
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Hybridization of bean, spinach, maize and Euglena chloroplast transfer RNAs with homologous and heterologous chloroplast DNAs. An approach to the study of homology between chloroplast tRNAs from various species.菜豆、菠菜、玉米和眼虫叶绿体转移RNA与同源及异源叶绿体DNA的杂交。一种研究不同物种叶绿体tRNA之间同源性的方法。
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Fractionation and identification of Euglena gracilis cytoplasmic and chloroplastic tRNAs and mapping of tRNA genes on chloroplast DNA.小球藻细胞质和叶绿体 tRNA 的分离与鉴定及叶绿体 DNA 上 tRNA 基因的定位。
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2
Chloroplast transfer RNAs and tRNA genes of wheat.小麦叶绿体转移 RNA 及其 tRNA 基因。
Plant Mol Biol. 1985 Sep;4(5):315-20. doi: 10.1007/BF02418251.
3
Distribution of transfer RNA genes in thePisum sativum chloroplast DNA.豌豆叶绿体 DNA 中转录 RNA 基因的分布。

本文引用的文献

1
A gene coding for tRNA is located near 5' terminus of 16S rRNA gene in Zea mays chloroplast genome.在玉米叶绿体基因组中,tRNA 基因编码位于 16S rRNA 基因的 5' 末端附近。
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4748-52. doi: 10.1073/pnas.78.8.4748.
2
Overlapping divergent genes in the maize chloroplast chromosome and in vitro transcription of the gene for tRNA.玉米叶绿体染色体中重叠的分歧基因和 tRNA 基因的体外转录。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3423-7. doi: 10.1073/pnas.78.6.3423.
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Endonuclease recognition sites mapped on Zea mays chloroplast DNA.
Plant Mol Biol. 1985 Mar;4(2-3):65-79. doi: 10.1007/BF02418752.
4
Organization and nucleotide sequence of the genes for spinach chloroplast tRNA(Glu) and tRNA (Tyr.).菠菜叶绿体 tRNA(Glu)和 tRNA(Tyr.)基因的组织和核苷酸序列。
Plant Mol Biol. 1984 Sep;3(5):313-7. doi: 10.1007/BF00017785.
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Mapping of transfer RNA genes on tobacco chloroplast DNA.烟草叶绿体 DNA 中转录 RNA 基因的定位。
Plant Mol Biol. 1984 Jan;3(1):29-36. doi: 10.1007/BF00023413.
6
DNA sequences for the Zea mays tRNA genes tV-UAC and tS-UGA: tV-UAC contains a large intron.玉米 tRNA 基因 tV-UAC 和 tS-UGA 的 DNA 序列:tV-UAC 含有一个大内含子。
Plant Mol Biol. 1984 Jan;3(1):13-20. doi: 10.1007/BF00023411.
7
Nucleotide sequences of transfer RNA genes in the Pisum sativum chloroplast DNA.豌豆叶绿体 DNA 中转录 RNA 基因的核苷酸序列。
Plant Mol Biol. 1986 Jan;6(1):1-12. doi: 10.1007/BF00021301.
8
The region distal to the rRNA operon from chloroplasts of maize contains genes coding for tRNA(Arg)(ACG) and tRNA (Asn)(GUU).来自玉米叶绿体 rRNA 操纵子远端的区域包含编码 tRNA(Arg)(ACG)和 tRNA (Asn)(GUU)的基因。
Plant Mol Biol. 1986 Nov;7(6):419-31. doi: 10.1007/BF00020326.
9
A model for the evolution of the Vicia faba chloroplast genome.蚕豆叶绿体基因组进化模型。
Theor Appl Genet. 1987 May;74(1):125-39. doi: 10.1007/BF00290095.
10
Transfer RNAs and tRNA genes of Vicia faba chloroplasts.蚕豆叶绿体的转移 RNA 和 tRNA 基因。
Curr Genet. 1984 Jul;8(5):379-85. doi: 10.1007/BF00419827.
绘制在玉米叶绿体DNA上的核酸内切酶识别位点
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4309-13. doi: 10.1073/pnas.73.12.4309.
4
Transcriptional origin of Euglena chloroplast tRNAs.眼虫叶绿体tRNA的转录起源
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1984-8. doi: 10.1073/pnas.73.6.1984.
5
Hybridization of bean, spinach, maize and Euglena chloroplast transfer RNAs with homologous and heterologous chloroplast DNAs. An approach to the study of homology between chloroplast tRNAs from various species.菜豆、菠菜、玉米和眼虫叶绿体转移RNA与同源及异源叶绿体DNA的杂交。一种研究不同物种叶绿体tRNA之间同源性的方法。
Biochim Biophys Acta. 1980 Aug 26;609(1):31-9. doi: 10.1016/0005-2787(80)90198-7.
6
Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes.对玉米叶绿体DNA核糖体RNA操纵子中的16S - 23S间隔区进行测序,发现了两个分裂的tRNA基因。
Cell. 1981 Jul;25(1):203-13. doi: 10.1016/0092-8674(81)90245-2.
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The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.编码于16S至23S核糖体DNA间隔区的玉米和菠菜叶绿体异亮氨酸转移RNA的核苷酸序列。
Nucleic Acids Res. 1982 Mar 11;10(5):1653-9. doi: 10.1093/nar/10.5.1653.
8
Nucleotide sequences of five maize chloroplast transfer RNA genes and their flanking regions.五个玉米叶绿体转运RNA基因及其侧翼区域的核苷酸序列。
J Biol Chem. 1983 May 10;258(9):5503-11.
9
Transfer RNA genes associated with the 16S and 23S rRNA genes of Euglena chloroplast DNA.与眼虫叶绿体DNA的16S和23S rRNA基因相关的转运RNA基因。
Biochem Biophys Res Commun. 1980 Jul 16;95(1):47-54. doi: 10.1016/0006-291x(80)90702-0.
10
Sequencing of 16S--23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genes.纤细裸藻叶绿体DNA核糖体RNA操纵子中16S - 23S间隔区的测序揭示了两个tRNA基因。
Nature. 1980 Aug 28;286(5776):908-10. doi: 10.1038/286908a0.