菜豆、菠菜、玉米和眼虫叶绿体转移RNA与同源及异源叶绿体DNA的杂交。一种研究不同物种叶绿体tRNA之间同源性的方法。

Hybridization of bean, spinach, maize and Euglena chloroplast transfer RNAs with homologous and heterologous chloroplast DNAs. An approach to the study of homology between chloroplast tRNAs from various species.

作者信息

Mubumbila M, Burkard G, Keller M, Steinmetz A, Crouse E, Weil J H

出版信息

Biochim Biophys Acta. 1980 Aug 26;609(1):31-9. doi: 10.1016/0005-2787(80)90198-7.

DOI:10.1016/0005-2787(80)90198-7

Abstract

Chloroplast tRNAs from two dicotyledons (spinach and bean), a monocotyledon (maize) and a green alga (Euglena) have been fractionated by two-dimensional gel electrophoresis. The individual tRNAs have been identified, albeled with 125I or 32P, and used in tRNA-DNA hybridization experiments. Spinach chloroplast tRNAs hybridize as well, and maize chloroplast tRNAs almost as well as bean chloroplast tRNAs to bean chloroplast DNA, thus suggesting a high degree of homology between the chloroplast tRNAs from the two dicotyledons and between the tRNAs from the two dicotyledons and those of the monocotyledon. But Euglena total chloroplast tRNA hybridizes very poorly to bean chloroplast DNA, and among the 14 individual tRNAs tested, only one, Euglena chloroplast tRNAPhe, hybridizes to both maize and bean chloroplast DNAs, which is in good agreement with the fact that Euglena and bean chloroplast tRNAsPhe have almost identical primary structures.

摘要

来自两种双子叶植物（菠菜和菜豆）、一种单子叶植物（玉米）和一种绿藻（眼虫藻）的叶绿体转运核糖核酸（tRNA）已通过双向凝胶电泳进行了分级分离。已对各个tRNA进行了鉴定，用¹²⁵I或³²P进行了标记，并用于tRNA-DNA杂交实验。菠菜叶绿体tRNA与菜豆叶绿体DNA的杂交效果良好，玉米叶绿体tRNA与菜豆叶绿体DNA的杂交效果几乎与菜豆叶绿体tRNA相同，这表明两种双子叶植物的叶绿体tRNA之间以及双子叶植物和单子叶植物的tRNA之间具有高度的同源性。但是眼虫藻的总叶绿体tRNA与菜豆叶绿体DNA的杂交效果非常差，在所测试的14种单个tRNA中，只有一种，即眼虫藻叶绿体tRNAPhe，能与玉米和菜豆的叶绿体DNA杂交，这与眼虫藻和菜豆叶绿体tRNAPhe具有几乎相同的一级结构这一事实高度吻合。