Pagé M, Dufour C
Biomedicine. 1976 Jul;25(5):170-3.
A spectrophotometric method for measuring mammalian cell growth in culture is described. The attached cells were solubilized in 2N sodium hydroxide and the absorption was measured at 225 nm. The technique is sensitive to about 3000 cells per Petri dish and a linear relationship was obtained between the number of cells and the absorption at this wave length. The method is rapid and the solubilized material can be recovered for liquid scintillation counting when needed.
本文描述了一种用于测量培养中的哺乳动物细胞生长的分光光度法。贴壁细胞用2N氢氧化钠溶解，并在225nm处测量吸光度。该技术对每个培养皿中约3000个细胞敏感，并且在该波长下细胞数量与吸光度之间获得了线性关系。该方法快速，并且在需要时可以回收溶解的物质用于液体闪烁计数。
