Zhang C X
Department of Biomaterials of Shanghai Second Medical University.
Zhonghua Kou Qiang Yi Xue Za Zhi. 1990 Jul;25(4):216-8, 252.
The authors developed a new measuring cytotoxic method for biomaterials using violet-spectrophotometer. The principle of this test is that the more the cell exist the higher the concentration of stain occur. Base this phenomena, we may calculate the relative growth rate and evaluated the toxic grade of the tested materials according to the absorption in spectrophotometer. The merits are that it can avoid subjective factor of the operator; collect all cells tested; be more sensitive, simple and accurate than the cell growth rate test, agar overlay test and molecular filter test. Three kinds of different cupric quantity amalgam are tested using new measuring cytotoxic method. The content of copper is 6%, 13% and 24.6% respectively. The result shows that there is no evident cytotoxicity for these three materials after 2 days, but the cytotoxicity appeared and increased as the contacting time between the extract of material and cell goes on. On the other hand, as the content of copper increased. The cell relative growth rate decreased. It indicated that there may be certain relationship between the content of copper and cell growth rate.
作者开发了一种使用紫外分光光度计测量生物材料细胞毒性的新方法。该测试的原理是细胞存在越多，染色浓度越高。基于这种现象，我们可以计算相对生长率，并根据分光光度计中的吸光度评估被测材料的毒性等级。其优点是可以避免操作人员的主观因素；收集所有被测细胞；比细胞生长率测试、琼脂覆盖测试和分子滤膜测试更灵敏、简单和准确。使用新的细胞毒性测量方法测试了三种不同铜含量的汞合金。铜含量分别为6%、13%和24.6%。结果表明，这三种材料在2天后没有明显的细胞毒性，但随着材料提取物与细胞接触时间的延长，细胞毒性出现并增加。另一方面，随着铜含量的增加，细胞相对生长率下降。这表明铜含量与细胞生长率之间可能存在一定关系。
