Meuret G, Bammert J, Gessner U
Blut. 1976 Dec;33(6):389-402. doi: 10.1007/BF00996571.
Neutrophil marrow egress is governed by several processes. The most important are cell maturation, functional behavior of marrow sinusoids and humoral or neuro-vascular factors. Neutrophil release cannot be observed directly but is reflected in the size, cellular composition and kinetics of the nonproliferating pool of granulocytopoiesis in bone marrow and of blood neutrophil pool. These experimentally determined parameters were used as the basis of a mathematical model study. The model describes two catenated compartments, the nonproliferating pool of granulocytopoiesis in marrow and the total blood granulocyte pool. Cell transit from one pool to the other was assumed to be age-dependent. It was expressed by a positive sloping sigmoidal function that defines the egress potential fo the cells that increases with cell maturation. During maturation granulocytopoietic cells develop intense motility which determines the morphology of the cells on smears. Relationship between cell motility and its morphology was defined by functions determining the age-dependent probabilities of cell fixation as metamyelocytes, band- and segmented forms, respectively. The parameters of this model could be so adjusted that all experimental data were matched within experimental errors. Thus, qualitative and quantitative information on neutrophil marrow egress was obtained for normal and pathological states of granulocytopoiesis.
中性粒细胞从骨髓中逸出受多种过程调控。其中最重要的是细胞成熟、骨髓血窦的功能行为以及体液或神经血管因子。中性粒细胞的释放无法直接观察到，但可通过骨髓中粒细胞生成的非增殖池以及血液中性粒细胞池的大小、细胞组成和动力学反映出来。这些通过实验确定的参数被用作数学模型研究的基础。该模型描述了两个相互关联的隔室，即骨髓中粒细胞生成的非增殖池和全血粒细胞池。假设细胞从一个池转移到另一个池与细胞年龄有关。它由一个正斜率的S形函数表示，该函数定义了随着细胞成熟而增加的细胞逸出潜能。在成熟过程中，粒细胞生成细胞产生强烈的运动性，这决定了涂片上细胞的形态。细胞运动性与其形态之间的关系分别由确定细胞固定为晚幼粒细胞、杆状核和分叶核形式的年龄依赖性概率的函数定义。该模型的参数可以进行调整，以使所有实验数据在实验误差范围内匹配。因此，获得了粒细胞生成正常和病理状态下中性粒细胞从骨髓中逸出的定性和定量信息。
