血清淀粉酶活性淀粉-碘测定法的干扰及高脂血症的影响。

Interferences with the starch-iodine assay for serum amylase activity, and effects of hyperlipemia.

作者信息

Gitlitz P H, Frings C S

出版信息

Clin Chem. 1976 Dec;22(12):2006-9.

Abstract

Recent reports imply or claim that amylase activity is inhibited in hyperlipemic sera, because diluted samples showed greater activities when assayed by a starch-iodine method. We find that dilutions of both clear and lipemic samples give higher-than-expected activities when assayed by a starch-iodine method, an effect attributable to the variable effects of protein, turbidity, and triglycerides in the hyperlipemic samples. Thus the starch-iodine method is unsuitable for assessing the effects of hyperlipemic samples on amylase activity. To do so, we used an alternative method, in which soluble dyed amylopectin is used as the substrate. This method exhibits apparent zero-order kinetics, and we detected no interfering factors. Plots of sample volume (x) vs. activity (y) for clear and hyperlipemic (triglycerides up to 80 g/liter) sera gave straight lines with y-intercepts of zero. Evidently hypertriglyceridemia does not inhibit amylase activity.

摘要

最近的报告暗示或声称，在高脂血症血清中淀粉酶活性受到抑制，因为用淀粉 - 碘法测定时，稀释后的样品显示出更高的活性。我们发现，无论是清亮样品还是脂血样品，用淀粉 - 碘法测定时，稀释后的活性都高于预期，这种效应可归因于高脂血症样品中蛋白质、浊度和甘油三酯的不同影响。因此，淀粉 - 碘法不适用于评估高脂血症样品对淀粉酶活性的影响。为此，我们采用了另一种方法，其中使用可溶性染色支链淀粉作为底物。该方法呈现明显的零级动力学，并且我们未检测到干扰因素。清亮血清和高脂血症（甘油三酯高达80克/升）血清的样品体积（x）与活性（y）的关系图给出了截距为零的直线。显然，高甘油三酯血症并不抑制淀粉酶活性。