Hatase O, Fujii T, Kuramitsu M, Itano T, Takahashi F, Murakami T, Nisida I
Acta Med Okayama. 1979 Apr;33(2):73-80.
Factors that inhibit and stimulate cell proliferation were found to coexist in rat liver supernatant. The inhibitory and stimulatory factors were separated by ethanol fractionation. Both factors were sensitive to heat- and trypsin-treatment. The activity of the inhibitor was diminished by partial hepatectomy. The inhibitor from normal livers inhibited DNA and RNA synthesis in the L-cell system, but the same fraction from regenerating livers caused little or no inhibition of nucleic acid synthesis. The stimulatory factor from regenerating livers had a stronger effect on cell proliferation than that of normal livers. Furthermore, the inhibitor from normal livers depressed DNA synthesis in vivo in regenerating livers.
人们发现，抑制和刺激细胞增殖的因子共存于大鼠肝脏上清液中。通过乙醇分级分离法将抑制因子和刺激因子分离开来。这两种因子对加热和胰蛋白酶处理均敏感。部分肝切除术后，抑制剂的活性降低。正常肝脏中的抑制剂可抑制L细胞系统中的DNA和RNA合成，但来自再生肝脏的相同组分对核酸合成几乎没有抑制作用。再生肝脏中的刺激因子对细胞增殖的作用比正常肝脏中的刺激因子更强。此外，正常肝脏中的抑制剂可抑制再生肝脏中体内的DNA合成。
