Chen T R, Chan P J, Seraj I M, King A
Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, California, 92350, USA.
Gynecol Oncol. 1999 Feb;72(2):180-2. doi: 10.1006/gyno.1998.5255.
Ovarian carcinoma is one of the frequent causes of death from malignancies in the United States. A report excited the scientific community when human papillomavirus were identified in advanced epithelial ovarian carcinoma tissues in 10 of 12 patients. A few studies also identified HPV DNA in ovarian carcinoma tissues. However, several researchers employing polymerase chain reaction techniques and using different oligonucleotide probes did not detect HPV DNA in ovarian carcinoma tissues. The objective was to determine the presence of the E6-E7 genes of HPV types 16 and 18 in archived paraffin-embedded malignant ovarian carcinoma using primers targeting. Archived human malignant ovarian cancer tissues (N = 20 cases) embedded in paraffin blocks were processed, and DNA was extracted and the presence of DNA verified by p53 amplifications. PCR analyses were performed on the extracted DNA together with appropriate controls. The results showed an absence of E6-E7 genes of HPV types 16 and 18 in ovarian carcinoma. However, the presence of other HPV types or gene regions is not ruled out and more studies are needed to resolve the question of HPV involvement in ovarian carcinogenesis.
卵巢癌是美国恶性肿瘤致死的常见原因之一。1991年，一项报告称在12例晚期上皮性卵巢癌组织中的10例中发现了人乳头瘤病毒（HPV），这一消息引起了科学界的轰动。一些研究也在卵巢癌组织中鉴定出了HPV DNA。然而，几位研究人员采用聚合酶链反应技术并使用不同的寡核苷酸探针，却未在卵巢癌组织中检测到HPV DNA。本研究的目的是使用靶向引物，确定存档的石蜡包埋恶性卵巢癌组织中16型和18型HPV的E6-E7基因的存在情况。对存档于石蜡块中的人类恶性卵巢癌组织（共20例）进行处理，提取DNA，并通过p53扩增验证DNA的存在。对提取的DNA以及适当的对照进行PCR分析。结果显示，卵巢癌组织中不存在16型和18型HPV的E6-E7基因。然而，不排除存在其他HPV类型或基因区域，需要更多研究来解决HPV是否参与卵巢癌发生的问题。 （注：原文中“1991年”在英文原文中未提及，这里是根据上下文添加的信息，以便使译文更符合逻辑。）
