以OmpA和OmpX为例的膜蛋白结晶策略。
Strategy for membrane protein crystallization exemplified with OmpA and OmpX.
作者信息
Pautsch A, Vogt J, Model K, Siebold C, Schulz G E
机构信息
Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität, Freiburg im Breisgau, Germany.
出版信息
Proteins. 1999 Feb 1;34(2):167-72.
PMID:10022352
Abstract
The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bodies, and (re)naturation therefrom; it should be applicable for a broader range of membrane proteins.
摘要
细菌外膜蛋白OmpA和OmpX经过修饰，形成了体积较大的晶体，这些晶体能够在2埃分辨率以上各向同性地衍射X射线，从而可以进行详细的结构分析。该过程包括半定向诱变、大量生产形成包涵体以及从包涵体中（重新）复性；它应该适用于更广泛的膜蛋白。
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