Matsuzaki T, Suzuki T, Koyama H, Tanaka S, Takata K
Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gunma University, Showa-machi 3-39-15, Maebashi, Gunma 371-8512, Japan.
Cell Tissue Res. 1999 Mar;295(3):513-21. doi: 10.1007/s004410051257.
Aquaporin-5 (AQP5) is a water channel protein and is considered to play an important role in water movement across the plasma membrane. We raised anti-AQP5 antibody and examined the localization of AQP5 protein in rat salivary and lacrimal glands by immunofluorescence microscopy. AQP5 was found in secretory acinar cells of submandibular, parotid, and sublingual glands, where it was restricted to apical membranes including intercellular secretory canaliculi. In the submandibular gland, abundant AQP5 was also found additionally at the apical membrane of intercalated duct cells. Upon stimulation by isoproterenol, apical staining for AQP5 in parotid acinar cells tended to appear as clusters of dots. These results suggest that AQP5 is one of the candidate molecules responsible for the water movement in the salivary glands.
水通道蛋白5（AQP5）是一种水通道蛋白，被认为在水跨质膜的转运过程中发挥重要作用。我们制备了抗AQP5抗体，并通过免疫荧光显微镜检查了AQP5蛋白在大鼠唾液腺和泪腺中的定位。在下颌下腺、腮腺和舌下腺的分泌性腺泡细胞中发现了AQP5，它局限于包括细胞间分泌小管在内的顶端膜。在下颌下腺中，闰管细胞的顶端膜上也额外发现了丰富的AQP5。经异丙肾上腺素刺激后，腮腺腺泡细胞中AQP5的顶端染色倾向于呈现为点状簇。这些结果表明，AQP5是负责唾液腺中水转运的候选分子之一。
