Cicchetti R, Bari M, Argentin G
Department of Public Health and Cell Biology, University of Rome 'Tor Vergata', Via di Tor Vergata 135, 00133, Rome, Italy.
Mutat Res. 1999 Feb 19;439(2):239-48. doi: 10.1016/s1383-5718(98)00185-5.
Two pesticides, organophosphate phosphamidon (PHO) and organochlorine dieldrin (DED) were assayed by the mouse bone marrow micronucleus test, to ascertain whether they showed genotoxic activity in vivo. Two doses, sub-lethal (PHO=3 mg/kg b.wt.; DED=60 mg/kg b.wt.) and lethal (PHO=5 mg/kg b.wt.; DED=90 mg/kg b.wt.), of each substance were administered intraperitoneally to 9-10-week old CBA male mice, in acute and repeated exposure. The sub-lethal dose was also administered at two different times and twice at 24-h intervals. Both PHO and DED proved able to induce a dose-dependent increase of micronucleated polychromatic erythrocytes (PCE). The two pesticides also showed a different detoxification time. Furthermore, the CREST staining with antikinetochore antibodies allowed us to conclude that the two chemicals are clastogens.
通过小鼠骨髓微核试验对两种农药进行了检测，这两种农药分别是有机磷农药磷胺（PHO）和有机氯农药狄氏剂（DED），目的是确定它们在体内是否具有遗传毒性。将每种物质的两个剂量，即亚致死剂量（PHO = 3毫克/千克体重；DED = 60毫克/千克体重）和致死剂量（PHO = 5毫克/千克体重；DED = 90毫克/千克体重），以急性和重复暴露的方式腹腔注射给9 - 10周龄的CBA雄性小鼠。亚致死剂量还在两个不同时间以24小时间隔给药两次。PHO和DED均被证明能够诱导微核多染性红细胞（PCE）呈剂量依赖性增加。这两种农药还表现出不同的解毒时间。此外，使用抗着丝粒抗体的CREST染色使我们能够得出结论，这两种化学物质都是断裂剂。
