Snarska Anna, Palus Katarzyna, Wysocka Dominika, Rytel Liliana
Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719, Olsztyn, Poland.
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719, Olsztyn, Poland.
J Vet Res. 2020 Oct 15;64(4):609-614. doi: 10.2478/jvetres-2020-0065. eCollection 2020 Dec.
Due to the widespread occurrence of acrylamide in the environment, its likely carcinogen status, and the suitability of the pig model as a human analogue, the authors decided to evaluate the impact of high and low doses of this compound on the processes of erythropoiesis in swine bone marrow.
The experiment was carried out on Danish Landrace pigs at the age of eight weeks and body weight about 20 kg. The animals were divided into three equal groups consisting of five pigs in each. Control animals received empty gelatin capsules (placebos). Animals from the first experimental group received a low dose of acrylamide of 0.5 μg/kg b.w./day (> 99% purity; Sigma-Aldrich, Poland), and animals from the second experimental group received a dose 10 times higher. Placebos and acrylamide capsules were administered with feed every morning for 28 days. After anaesthetisation of the animals, bone marrow from the femur was collected into tubes without an anticoagulant on days 0 and 28. After drying and staining, bone marrow smears were subjected to detailed cytological evaluation using a light microscope.
This study showed that high and low doses of acrylamide affected the process of porcine erythropoiesis. The cytotoxic effect of acrylamide on this process was demonstrated in a change of the polychromatic erythroblasts/normochromatic erythroblasts ratio.
Both doses of acrylamide caused a decrease in the number of ortho- and polychromatic erythroblasts.
由于丙烯酰胺在环境中广泛存在，其可能的致癌状态，以及猪模型作为人类类似物的适用性，作者决定评估高剂量和低剂量该化合物对猪骨髓红细胞生成过程的影响。
实验在8周龄、体重约20 kg的丹麦长白猪身上进行。动物被分为三组，每组五只。对照动物接受空明胶胶囊（安慰剂）。第一实验组的动物接受低剂量的丙烯酰胺，剂量为0.5 μg/kg体重/天（纯度>99%；Sigma-Aldrich，波兰），第二实验组的动物接受的剂量是其10倍。安慰剂和丙烯酰胺胶囊每天早上与饲料一起投喂，持续28天。在动物麻醉后，于第0天和第28天从股骨采集骨髓到无抗凝剂的试管中。干燥和染色后，使用光学显微镜对骨髓涂片进行详细的细胞学评估。
本研究表明，高剂量和低剂量的丙烯酰胺均影响猪的红细胞生成过程。丙烯酰胺对该过程的细胞毒性作用通过多色成红细胞/正色成红细胞比例的变化得以证明。
两种剂量的丙烯酰胺均导致正染和多染成红细胞数量减少。
