Moryś J, Berdel B, Kowiański P, Majak K, Tarnawski M, Wisniewski H M
Department of Anatomy and Neurobiology, Medical University of Gdańsk, Poland.
Neurosci Lett. 1999 Jan 8;259(2):91-4. doi: 10.1016/s0304-3940(98)00908-2.
Two-laser and two-color approaches were used to observe the colocalization of the calcium-binding proteins, calbindin D28k and parvalbumin, and the retrograde tracer, Fluoro-Gold (FG) in the basolateral amygdala of the rat. The study was performed on five adult rats into which FG was injected to the frontal association cortex. Then, the localization of the retrogradely labeled neurons in the basolateral amygdala was compared with the localization of the neurons labeled by calcium-binding proteins. The present study showed that most of the retrogradely labeled neurons in the posterior part of the basolateral amygdala are also calbindin-positive. Even though a lot of parvalbumin-positive endings were present at the surface of the retrogradely labeled cells, we did not observe the colocalization of the parvalbumin and projective neurons.
采用双激光双色方法观察大鼠基底外侧杏仁核中钙结合蛋白钙结合蛋白D28k和小白蛋白与逆行示踪剂氟金（FG）的共定位。对5只成年大鼠进行了该研究，将FG注入额叶联合皮层。然后，将基底外侧杏仁核中逆行标记神经元的定位与钙结合蛋白标记神经元的定位进行比较。本研究表明，基底外侧杏仁核后部大多数逆行标记神经元也为钙结合蛋白阳性。尽管在逆行标记细胞表面存在许多小白蛋白阳性终末，但我们未观察到小白蛋白与投射神经元的共定位。
