Risold P Y, Griffond B, Kilduff T S, Sutcliffe J G, Fellmann D
CNRS ESA 6025, Laboratoire d'Histologie-Embryologie-Cytogénétique, Institut d'Etudes et de Transfert de Gènes, Faculté de Médecine, Besançon, France.
Neurosci Lett. 1999 Jan 15;259(3):153-6. doi: 10.1016/s0304-3940(98)00906-9.
Recently, two new neuropeptides named hypocretins I and II (Hcrt) have been described in the rat lateral hypothalamus. The distribution of the neurons expressing these new peptides is similar to that of neurons described in the literature as prolactin (PRL) immunoreactive neurons and specifically labeled by an antiserum (AS) raised against ovine prolactin (oPRL). In this study, we report that every neuron labeled by immunohistochemistry or in situ hybridization for Hcrt is also labeled by the oPRL-AS, and conversely. Furthermore, the labeling of the oPRL-AS is inhibited when this AS is pre-incubated with the 104-109 fragment of the preproHcrt, thus indicating that the oPRL-AS is recognizing an epitope carried by this particular fragment of the preproHcrt. Our previous experimental work on these neurons showed that they may be involved in energy metabolism and water balance, which is in agreement with the current literature about Hcrt functions.
最近，在大鼠下丘脑外侧区发现了两种新的神经肽，即食欲素I和II（Hcrt）。表达这些新肽的神经元分布，与文献中所述的催乳素（PRL）免疫反应性神经元的分布相似，并且能被一种针对羊催乳素（oPRL）产生的抗血清（AS）特异性标记。在本研究中，我们报告称，通过免疫组织化学或原位杂交标记的每一个Hcrt神经元，也都被oPRL-AS标记，反之亦然。此外，当该AS与前食欲素原的104-109片段预孵育时，oPRL-AS的标记会受到抑制，这表明oPRL-AS识别的是前食欲素原这一特定片段携带的一个表位。我们之前对这些神经元的实验研究表明，它们可能参与能量代谢和水平衡，这与目前关于Hcrt功能的文献一致。
