de Vries G P, Meijer A E
Histochemistry. 1976 Nov 19;50(1):1-8. doi: 10.1007/BF00492780.
Improved histochemical multi-step techniques for the demonstration of glucose 6-phosphate isomerase and phosphoglucomutase in tissue sections are described. With these techniques a semipermeable membrane is interposed between the incubating solutions and the tissue sections preventing diffusion of enzymes into the medium during incubation. In the histochemical system the glucosephosphate isomerase converts the substrate D-fructo-furanose 6-phosphoric acid to D-gluco-pyranose 6-phosphoric acid, and the phosphoglucomutase converts the substrate alpha-D-glucose 1-phosphate to the same reagent, which in turn is oxidized, by exogenous and endogenous glucose 6-phosphate dehydrogenase to D-glucono-delta-lactone 6-phosphoric acid. Concomittantly the electrons are transferred via NADP+, phenazine methosulphate and menadione to nitro-BT. Sodiumazide and amytal are incorporated to block electron transfer to the cytochromes.
本文描述了用于在组织切片中显示葡萄糖-6-磷酸异构酶和磷酸葡萄糖变位酶的改良组织化学多步技术。通过这些技术，在孵育溶液和组织切片之间插入半透膜，防止酶在孵育过程中扩散到培养基中。在组织化学系统中，葡萄糖磷酸异构酶将底物D-果糖呋喃糖6-磷酸转化为D-葡萄糖吡喃糖6-磷酸，磷酸葡萄糖变位酶将底物α-D-葡萄糖1-磷酸转化为相同试剂，该试剂又被外源性和内源性葡萄糖-6-磷酸脱氢酶氧化为D-葡萄糖酸-δ-内酯6-磷酸。同时，电子通过NADP +、吩嗪硫酸甲酯和甲萘醌转移至硝基蓝四氮唑。加入叠氮化钠和戊巴比妥以阻断电子向细胞色素的转移。
