Elias E A, De Vries G P, Elias R A, Tigges A J, Meijer A E
Histochem J. 1980 Sep;12(5):577-89. doi: 10.1007/BF01011931.
In this communication, the results of applying various histochemical techniques for the localization of oxidoreductases, transferases, hydrolases and isomerases in the human heart are presented. The Purkinje fibres of the atrioventricular conducting system of the human heart differ from the myocardium proper in containing a slightly higher activity of most of the glycolytic and gluconeogenetic enzymes investigated. The relatively higher activity of 6-phosphofructokinase, the key enzyme in anaerobic carbohydrate metabolism, is especially noteworthy. On the other hand, the activities of some of the enzymes that play a part in the aerobic energy metabolism is slightly less than those in the myocardium fibres. As for the activity of the NADPH regenerating enzymes, the activity of 6-phosphogluconate dehydrogenase and malate dehydrogenase (oxaloacetate-decarboxylating) is somewhat higher, and the activity of glucose-6-phosphate dehydrogenase similar, in the Purkinje fibres compared to that in the myocardial fibres. The activity of myosin ATPase is similar for both types of fibre. Likewise, the fibres of the conducting system and of the myocardium show a similar activity of acid phosphatase, beta-glucuronidase, non-specific naphthylesterase and peroxidase. The neurogenic function of the conducting system of the human heart was demonstrated by the high activity of acetylcholinesterase in the Purkinje fibres and in the atrioventricular node. All these histochemical findings in Purkinje fibres are similar at widely differing levels of the conducting system.
在本报告中，展示了应用各种组织化学技术对人心脏中的氧化还原酶、转移酶、水解酶和异构酶进行定位的结果。人心脏房室传导系统的浦肯野纤维与心肌本身不同，在所研究的大多数糖酵解和糖异生酶中，其活性略高。尤其值得注意的是，厌氧碳水化合物代谢中的关键酶6-磷酸果糖激酶的活性相对较高。另一方面，一些参与有氧能量代谢的酶的活性略低于心肌纤维中的活性。至于NADPH再生酶的活性，与心肌纤维相比，浦肯野纤维中6-磷酸葡萄糖酸脱氢酶和苹果酸脱氢酶（草酰乙酸脱羧）的活性略高，葡萄糖-6-磷酸脱氢酶的活性相似。两种类型的纤维中肌球蛋白ATP酶的活性相似。同样，传导系统和心肌的纤维在酸性磷酸酶、β-葡萄糖醛酸酶、非特异性萘酯酶和过氧化物酶方面表现出相似的活性。人心脏传导系统的神经源性功能通过浦肯野纤维和房室结中乙酰胆碱酯酶的高活性得以证明。在传导系统的广泛不同水平上，浦肯野纤维中的所有这些组织化学发现都是相似的。
