Meijer A E, Vries G P
Histochem J. 1978 Jul;10(4):399-408. doi: 10.1007/BF01003003.
In this communication the results of applying various histochemical semipermeable membrane techniques to the localization of several enzymes in bovine and porcine heart are presented. The Purkinje fibers of the atrioventricular conducting system of the bovine heart differ from the myocardium proper in containing a greater activity of the glycolytic and gluconeogenetic enzymes--lactate dehydrogenase, glyceraldehyde-phosphate dehydrogenase, hexokinase, glucosephosphate isomerase and phosphoglucomutase, and less activity of the aerobic enzymes--NADH: nitroBT oxidoreductase and isocitrate dehydrogenase (NADP+). The metabolic reactions obtained with Purkinje fibers of the porcine heart are less pronounced. These histochemical findings are in accordance with the impression that Purkinje fibers, compared with the common myocardial fibers, have a higher rate of anaerobic metabolism and a lower rate of aerobic metabolism. The activity of the NADPH regenerating enzymes glucose-6-phosphate dehydrogenase and phosphogluconate dehydrogenase (decarboxylating), and the activity of acid hydrolases such as non-specific esterase and acid phosphatase is higher in the Purkinje fibers of both the bovine and porcine heart.
本文介绍了将各种组织化学半透膜技术应用于牛和猪心脏中几种酶定位的结果。牛心脏房室传导系统的浦肯野纤维与心肌本身不同，其糖酵解和糖异生酶（乳酸脱氢酶、磷酸甘油醛脱氢酶、己糖激酶、磷酸葡萄糖异构酶和磷酸葡萄糖变位酶）活性更高，而需氧酶（NADH：硝基BT氧化还原酶和异柠檬酸脱氢酶（NADP +））活性更低。猪心脏浦肯野纤维获得的代谢反应不太明显。这些组织化学发现与以下观点一致：与普通心肌纤维相比，浦肯野纤维具有更高的无氧代谢率和更低的有氧代谢率。牛和猪心脏的浦肯野纤维中，NADPH再生酶葡萄糖-6-磷酸脱氢酶和磷酸葡萄糖酸脱氢酶（脱羧）的活性以及酸性水解酶如非特异性酯酶和酸性磷酸酶的活性更高。
