前列腺素EP3受体在大鼠下丘脑的免疫细胞化学定位
Immunocytochemical localization of prostaglandin EP3 receptor in the rat hypothalamus.
作者信息
Nakamura K, Kaneko T, Yamashita Y, Hasegawa H, Katoh H, Ichikawa A, Negishi M
机构信息
Department of Molecular Neurobiology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
出版信息
Neurosci Lett. 1999 Jan 29;260(2):117-20. doi: 10.1016/s0304-3940(98)00962-8.
A rabbit antibody against an N-terminal portion of rat prostaglandin EP3 receptor (EP3R) was produced to examine the distribution of EP3R in the rat hypothalamus. The antibody specifically recognized EP3R proteins in rat brain extract, in membrane fractions of rat kidney, and in membrane fractions of EP3R-expressing culture cells. Intense EP3R-like immunoreactivity was observed in the median preoptic nucleus, medial preoptic area, parastrial nucleus, compact part of the dorsomedial hypothalamic nucleus, and dorsal part of the premammillary nucleus. These results suggest that prostaglandin E2 mediates various actions in the hypothalamus, such as fever induction in the preoptic area, through EP3R.
制备了一种针对大鼠前列腺素EP3受体（EP3R）N端部分的兔抗体，以检测EP3R在大鼠下丘脑的分布。该抗体能特异性识别大鼠脑提取物、大鼠肾脏膜组分以及表达EP3R的培养细胞膜组分中的EP3R蛋白。在视前正中核、视前内侧区、室旁核、下丘脑背内侧核致密部和乳头体前核背侧部观察到强烈的EP3R样免疫反应性。这些结果表明，前列腺素E2通过EP3R在下丘脑中介导多种作用，如在视前区诱导发热。
Zotero 插件