Lavrentieva I, Broude N E, Lebedev Y, Gottesman I I, Lukyanov S A, Smith C L, Sverdlov E D
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
FEBS Lett. 1999 Jan 29;443(3):341-7. doi: 10.1016/s0014-5793(99)00004-6.
The polymorphism at the multitude of loci adjacent to human endogenous retrovirus long terminal repeats (LTRs) was analyzed by a technique for whole genome differential display based on the PCR suppression effect that provides selective amplification and display of genomic sequences flanking interspersed repeated elements. This strategy is simple, target-specific, requires a small amount of DNA and provides reproducible and highly informative data. The average frequency of polymorphism observed in the vicinity of the LTR insertion sites was found to be about 12%. The high incidence of polymorphism within the LTR flanks together with the frequent location of LTRs near genes makes the LTR loci a useful source of polymorphic markers for gene mapping.
采用一种基于PCR抑制效应的全基因组差异显示技术，分析了人类内源性逆转录病毒长末端重复序列（LTR）相邻众多位点的多态性，该技术可对散布重复元件两侧的基因组序列进行选择性扩增和显示。此策略简单、具有靶点特异性，所需DNA量少，并能提供可重复且信息丰富的数据。发现在LTR插入位点附近观察到的多态性平均频率约为12%。LTR侧翼内多态性的高发生率以及LTR在基因附近的频繁定位，使得LTR位点成为基因定位多态性标记的有用来源。
