Ebert E C
Department of Medicine,UMDNJ- Robert Wood Johnson Medical School, One Robert Wood Johnson Place, New Brunswick, NJ 08903, USA.
Gut. 1999 Mar;44(3):342-6. doi: 10.1136/gut.44.3.342.
Murine intraepithelial lymphocytes kill Giardia lambia; responses of human intestinal lymphocytes to this parasite are unknown.
To examine giardia induced proliferation, interferon gamma production, migration, and cytotoxicity by lymphocytes from the human intestine and peripheral blood.
Giardia were added to intraepithelial lymphocytes, lamina propria lymphocytes, and peripheral blood lymphocytes, obtained from jejunal mucosa and blood of otherwise healthy patients undergoing gastric bypass surgery for morbid obesity. Proliferation was measured by 3H-thymidine incorporation; frequency of proliferation precursors, by limiting dilution analysis; interferon gamma production, by ELISA; cytotoxicity, by 51Cr release of radiolabelled giardia and by release of serine esterases by effector lymphocytes that mediate cytotoxicity.
The CD4+ T lymphocytes from intestine and blood proliferated in response to giardia. The stimulus by the parasite was mitogenic rather than antigenic due to the fact that the peak response was on day 3 rather than day 6, and the large number of precursors was in the range of that for mitogens. CD4+ T lymphocytes from both sites produced interferon gamma in response to giardia. Lymphocytes did not migrate towards or kill the parasite.
Giardia induced the same degree of proliferation and interferon gamma production by CD4+ T lymphocytes in intestine and blood, but did not trigger cytotoxicity or migration.
小鼠上皮内淋巴细胞可杀死蓝氏贾第鞭毛虫；人类肠道淋巴细胞对该寄生虫的反应尚不清楚。
研究蓝氏贾第鞭毛虫对人肠道和外周血淋巴细胞增殖、γ干扰素产生、迁移及细胞毒性的诱导作用。
将蓝氏贾第鞭毛虫加入从接受病态肥胖胃旁路手术的健康患者的空肠黏膜和血液中获取的上皮内淋巴细胞、固有层淋巴细胞及外周血淋巴细胞中。通过³H-胸腺嘧啶核苷掺入法检测增殖；通过有限稀释分析检测增殖前体细胞频率；通过酶联免疫吸附测定法检测γ干扰素产生；通过放射性标记的蓝氏贾第鞭毛虫的⁵¹Cr释放及介导细胞毒性的效应淋巴细胞释放丝氨酸酯酶检测细胞毒性。
来自肠道和血液的CD4⁺T淋巴细胞对蓝氏贾第鞭毛虫有增殖反应。由于峰值反应出现在第3天而非第6天，且大量前体细胞处于促有丝分裂原的范围内，因此寄生虫的刺激是有丝分裂原性而非抗原性的。来自这两个部位的CD4⁺T淋巴细胞对蓝氏贾第鞭毛虫均产生γ干扰素。淋巴细胞不会向寄生虫迁移或杀死寄生虫。
蓝氏贾第鞭毛虫在肠道和血液中诱导CD4⁺T淋巴细胞产生相同程度的增殖和γ干扰素，但不会引发细胞毒性或迁移。
