Yamagata M, Mori M, Mimori K, Mafune K I, Tanaka Y, Ueo H, Akiyoshi T
Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
Br J Cancer. 1999 Feb;79(3-4):565-9. doi: 10.1038/sj.bjc.6690089.
To clarify the significance of the expression of pyrimidine nucleoside phosphorylase (PyNPase) mRNA as a predictive factor for the prognosis of patients with oesophageal carcinoma, the PyNPase mRNA in the tumours and normal tissues from 55 resected cases of oesophageal carcinoma was examined by a reverse transcription polymerase chain reaction (RT-PCR). As a result, a positive correlation was observed between the tumour/normal (T/N) ratio of the expression of PyNPase mRNA by RT-PCR and that of the enzyme activity of PyNPase based on the findings of an enzyme linked immunosolvent assay (r = 0.594, P = 0.009). The T/N ratio of the expression of PyNPase mRNA was significantly higher in the cases with lymph vessel invasion (P = 0.013), lymph node metastasis (P = 0.0016), and an advanced stage of the disease (P = 0.021) than those without these factors. The patients with a higher T/N ratio of PyNPase mRNA showed significantly worse prognosis than those with a lower T/N ratio (P = 0.023 with log-rank tests). A multivariate analysis for the cumulative survival rates revealed that a high T/N ratio of the expression of PyNPase mRNA was independently related to a poor prognosis. These findings suggested that the determination of PyNPase mRNA by RT-PCR thus appears to be a new useful parameter for identifying both a poor prognosis and a highly malignant potential of oesophageal carcinoma.
为阐明嘧啶核苷磷酸化酶（PyNPase）mRNA的表达作为食管癌患者预后预测因子的意义，采用逆转录聚合酶链反应（RT-PCR）检测了55例手术切除的食管癌患者肿瘤组织和正常组织中的PyNPase mRNA。结果显示，基于酶联免疫吸附测定结果，RT-PCR检测的PyNPase mRNA表达的肿瘤/正常（T/N）比值与PyNPase酶活性的T/N比值之间呈正相关（r = 0.594，P = 0.009）。有淋巴管浸润（P = 0.013）、淋巴结转移（P = 0.0016）和疾病晚期（P = 0.021）的患者，PyNPase mRNA表达的T/N比值显著高于无这些因素的患者。PyNPase mRNA的T/N比值较高的患者预后明显比T/N比值较低的患者差（对数秩检验，P = 0.023）。对累积生存率的多因素分析显示，PyNPase mRNA表达的高T/N比值与预后不良独立相关。这些发现表明，通过RT-PCR检测PyNPase mRNA似乎是识别食管癌预后不良和高恶性潜能的一个新的有用参数。
