Labrenz M, Collins M D, Lawson P A, Tindall B J, Schumann P, Hirsch P
Institut für Allgemeine Mikrobiologie, Universität Kiel, Germany.
Int J Syst Bacteriol. 1999 Jan;49 Pt 1:137-47. doi: 10.1099/00207713-49-1-137.
Eight Gram-negative, aerobic, pointed and budding bacteria were isolated from various depths of the hypersaline, heliothermal and meromictic Ekho Lake (Vestfold Hills, East Antarctica). The cells contained storage granules and daughter cells could be motile. Bacteriochlorophyll a was sometimes produced, but production was repressed by constant dim light. The strains tolerated a wide range of temperature, pH, concentrations of artificial seawater and NaCl, but had an absolute requirement for sodium ions. Glutamate was metabolized with and without an additional source of combined nitrogen. The dominant fatty acid was C18:1; other characteristic fatty acids were C18:2, C12:0 2-OH, C12:1 3-OH, C16:1, C16:0 and C18:0. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G+C base composition was 62-64 mol%. 16S rRNA gene sequence comparisons showed that the isolates were phylogenetically close to the genera Antarctobacter, 'Marinosulfonomonas', Octadecabacter, Sagittula, Sulfitobacter and Roseobacter. Morphological, physiological and genotypic differences to these previously described and distinct genera support the description of a new genus and a new species, Roseovarius tolerans gen. nov., sp. nov. The type strain is EL-172T (= DSM 11457T).
从高盐、高温且水体存在化学分层的埃克霍湖（南极东部韦斯特福尔德丘陵）不同深度分离出8株革兰氏阴性、需氧、具尖状且出芽的细菌。细胞含有贮藏颗粒，子细胞具有运动性。有时会产生细菌叶绿素a，但持续的弱光会抑制其产生。这些菌株能耐受较宽的温度、pH值、人工海水和氯化钠浓度范围，但对钠离子有绝对需求。在有或没有额外化合态氮源的情况下，谷氨酸都能被代谢。主要脂肪酸为C18:1；其他特征性脂肪酸为C18:2、C12:0 2-OH、C12:1 3-OH、C16:1、C16:0和C18:0。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰胆碱。DNA的G+C碱基组成为62 - 64 mol%。16S rRNA基因序列比较表明，这些分离株在系统发育上与南极杆菌属、“海洋磺基单胞菌属”、十八杆菌属、箭菌属、亚硫酸还原菌属和玫瑰杆菌属相近。与这些先前描述的不同属在形态、生理和基因型上的差异支持了一个新属和一个新种的描述，即耐盐玫瑰变色菌属（Roseovarius tolerans），新属，新种。模式菌株为EL-172T（= DSM 11457T）。
