Behrendt U, Ulrich A, Schumann P, Erler W, Burghardt J, Seyfarth W
Centre for Agricultural Landscape and Land Use Research (ZALF), Institute of Microbial Ecology and Soil Biology, Müncheberg, Germany.
Int J Syst Bacteriol. 1999 Jan;49 Pt 1:297-308. doi: 10.1099/00207713-49-1-297.
The taxonomic position of a yellow-pigmented group of bacteria, isolated from the phyllosphere of grasses was investigated. Results obtained from restriction analysis of amplified 16S rDNA with seven endonucleases (CfoI, HaeIII, AluI, HinfI, MspI, Sau3A and ScrFI) showed identical restriction patterns for each enzyme of all isolates studied, which suggests that all strains belong to the same species. The grass isolates displayed the characteristics of the genus Pseudomonas. They were Gram-negative, aerobic and rod-shaped with polar flagella. Isolates were catalase-positive and oxidase-negative, and unable to oxidize or ferment glucose with the production of acid. The isolates did not reduce nitrate to nitrite but were able to utilize a wide range of compounds individually as a sole carbon source, with preference being given to the utilization of monosaccharides. The disaccharides tested were not utilized as substrates. The DNA base compositions of the tested strains ranged from 60 to 61 mol% G+C. The major isoprenoid quinone of each was ubiquinone Q-9 and hydroxy fatty acids were represented by 3-hydroxydodecanoic acid and 2-hydroxydodecanoic acid. Comparison of 16S rDNA sequences showed that the bacteria were members of the genus Pseudomonas, with similarity values between 91.5 and 97.7%. DNA-DNA hybridization studies with closely related neighbours revealed a low level of homology (< 27%), indicating that the isolates represent an individual species. On the basis of phenotypic and phylogenetic analyses a new species, Pseudomonas graminis sp. nov. (type strain DSM 11363T), is proposed.
对从禾本科植物叶际分离出的一组黄色色素细菌的分类地位进行了研究。用七种核酸内切酶（CfoI、HaeIII、AluI、HinfI、MspI、Sau3A和ScrFI）对扩增的16S rDNA进行限制性分析，结果表明，所有研究的分离株对每种酶都显示出相同的限制性图谱，这表明所有菌株都属于同一物种。禾本科植物分离株表现出假单胞菌属的特征。它们为革兰氏阴性、需氧、杆状，具极生鞭毛。分离株过氧化氢酶阳性、氧化酶阴性，不能氧化或发酵葡萄糖产酸。分离株不能将硝酸盐还原为亚硝酸盐，但能够单独利用多种化合物作为唯一碳源，优先利用单糖。所测试的双糖不能作为底物利用。测试菌株的DNA碱基组成为60至61 mol% G+C。每种菌株的主要类异戊二烯醌为泛醌Q-9，羟基脂肪酸以3-羟基十二烷酸和2-羟基十二烷酸为代表。16S rDNA序列比较表明，这些细菌是假单胞菌属的成员，相似性值在91.5%至97.7%之间。与近缘菌株进行的DNA-DNA杂交研究显示同源性水平较低（<27%），表明这些分离株代表一个独立的物种。基于表型和系统发育分析，提出了一个新物种，即禾本科假单胞菌（Pseudomonas graminis sp. nov.）（模式菌株DSM 11363T）。
