García-Castiñeiras S
Departamentos de Oftalmologia y Bioquímica, Escuela de Medicina, Universidad de Puerto Rico, San Juan.
P R Health Sci J. 1998 Dec;17(4):335-43.
Ideas prevailing in 1991 on hydrogen peroxide in the aqueous humor are outlined. They are critically examined under the light of our finding that the method used to establish aqueous humor levels of peroxide generates itself peroxide during the short time span of the analysis. This is due to the fact that the probe used, dichlorophenol indophenol (DCPIP), spontaneously auto-oxidizes in the presence of oxygen. It was concluded then that the level of hydrogen peroxide in the aqueous humor cannot be higher than about 0.3 microM, the detection limit of the DCPIP method. It was also concluded that the statement commonly made in the literature that aqueous humor hydrogen peroxide derives from the oxidation of ascorbate, an abundant component of that fluid, is based solely on the use of the DCPIP method, and so could easily be due to a methodological artifact. The same applies to the statement that the levels of hydrogen peroxide are very high in human senile cataracts. The surprising resistance to accept the results and conclusions of our 1992 publication is documented. Finally, the content is discussed of an oral presentation made at the 1997 ARVO Annual Meeting in which an important portion of our results and conclusions was confirmed, perhaps signaling a shift towards a wider acceptance of our findings.
概述了1991年关于房水中过氧化氢的主流观点。鉴于我们的发现，即用于测定房水中过氧化物水平的方法在分析的短时间内自身会产生过氧化物，对这些观点进行了批判性审视。这是因为所使用的探针二氯酚靛酚（DCPIP）在有氧存在的情况下会自发自动氧化。当时得出的结论是，房水中过氧化氢的水平不可能高于约0.3微摩尔，这是DCPIP方法的检测限。还得出结论，文献中普遍提到的房水中过氧化氢源自该液体中丰富成分抗坏血酸的氧化这一说法，仅仅是基于DCPIP方法的使用，因此很可能是由于方法学假象。关于人类老年性白内障中过氧化氢水平非常高的说法同样如此。记录了对我们1992年出版物的结果和结论令人惊讶的抵制情况。最后，讨论了在1997年视觉与眼科学研究协会（ARVO）年会上的一次口头报告内容，其中我们的部分结果和结论得到了证实，这或许标志着朝着更广泛接受我们的发现转变的一个信号。
