Bleau G, Giasson C, Brunette I
Department of Obstetrics and Gynecology, University of Montreal, Montreal, Quebec, Canada.
Anal Biochem. 1998 Oct 1;263(1):13-7. doi: 10.1006/abio.1998.2801.
The physiological concentration of hydrogen peroxide in the aqueous humor was reported to range between 25 and 60 microM, and conditions leading to elevated levels could have important damaging effects such as cataract formation. However, the high concentration of ascorbic acid in aqueous humor, which is 20 times that of plasma, was recently shown to interfere in the dichlorophenol-indophenol assay for hydrogen peroxide. The actual concentration of hydrogen peroxide in this fluid has become a controversial issue. In the present study, we used the method of ferrous oxidation of xylenol orange (FOX1 assay) performed in a nitrogen atmosphere to accurately measure low levels of hydrogen peroxide, even in the presence of ascorbic acid at concentrations normally found in aqueous humor. Contrary to values reported in the literature, we observed that the concentration of hydrogen peroxide in the rabbit aqueous humor is less than 5 microM, which is the detection limit of the method.
据报道，房水中过氧化氢的生理浓度在25至60微摩尔之间，导致其水平升高的情况可能会产生重要的损害作用，如白内障形成。然而，房水中高浓度的抗坏血酸（其浓度是血浆的20倍）最近被证明会干扰用于检测过氧化氢的二氯酚靛酚测定法。这种液体中过氧化氢的实际浓度已成为一个有争议的问题。在本研究中，我们采用在氮气氛围中进行的二甲酚橙亚铁氧化法（FOX1测定法）来准确测量低水平的过氧化氢，即使存在房水中通常浓度的抗坏血酸。与文献报道的值相反，我们观察到兔房水中过氧化氢的浓度低于5微摩尔，这是该方法的检测限。
