Yamamura H, Hiraide A, Matsuoka T, Takaoka M, Shimazu T, Sugimoto H
Department of Traumatology, Osaka University Medical School, Japan.
J Trauma. 1999 Feb;46(2):292-6. doi: 10.1097/00005373-199902000-00016.
Because of the known sodium and water retention associated with growth hormone (GH) therapy, it is crucial to evaluate the safety of GH after brain injury. To clarify this issue, we investigated whether GH affects brain edema in a rat brain freeze-injury model.
Male Wistar rats (n = 29) were divided into four groups according to the substance injected (GH vs. normal saline) and whether the brain was injured or not. The subcutaneous injections of GH (0.8 IU/kg) or saline were given 24 hours apart. In the injury groups, after the second injection, an aluminum rod (4-mm diameter) cooled to -50 degrees C was placed on the exposed dura mater in the right parietal region for 4 minutes, under anesthesia. At 4 hours after the insult, brain and skeletal muscle were excised and their water content was measured by drying.
Freeze injury of the brain caused an increase in water and sodium content in skeletal muscle. GH injection augmented this edema in skeletal muscle. Freeze injury of the brain also caused an increase in water and sodium content in the injured hemisphere of the brain. GH injection did not exacerbate this edema in injured brain tissue. Neither freeze injury nor GH injection caused brain edema in the noninjured hemisphere or in the cerebellum.
GH administration did not augment brain edema caused by brain injury in our model.
鉴于已知生长激素（GH）治疗会导致钠和水潴留，评估脑损伤后使用GH的安全性至关重要。为阐明这一问题，我们在大鼠脑冷冻损伤模型中研究了GH是否会影响脑水肿。
将29只雄性Wistar大鼠根据注射物质（GH与生理盐水）以及脑部是否受伤分为四组。GH（0.8 IU/kg）或生理盐水皮下注射，间隔24小时。在损伤组中，第二次注射后，在麻醉状态下将一根冷却至-50摄氏度、直径4毫米的铝棒置于右侧顶叶区域暴露的硬脑膜上4分钟。损伤后4小时，切除脑和骨骼肌，通过干燥测量其含水量。
脑冷冻损伤导致骨骼肌中水和钠含量增加。注射GH加剧了骨骼肌中的这种水肿。脑冷冻损伤还导致损伤半球的脑内水和钠含量增加。注射GH并未加剧损伤脑组织中的这种水肿。冷冻损伤和注射GH均未在未损伤半球或小脑中引起脑水肿。
在我们的模型中，给予GH并未加剧脑损伤引起的脑水肿。
