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在无场条件下饲养的果蝇幼虫中,关于低频电磁场对细胞转录影响的观察。

Observations on the effects of low frequency electromagnetic fields on cellular transcription in Drosophila larvae reared in field-free conditions.

作者信息

Tipping D R, Chapman K E, Birley A J, Anderson M

机构信息

School of Biological Sciences, University of Birmingham, Edgbaston, UK.

出版信息

Bioelectromagnetics. 1999;20(2):129-31.

Abstract

Drosophila larvae reared inside a micro-metal box with an internal field strength 0.004 microT, were treated with a magnetic field of 50 Hz, 8 microT. for 20 min. Control experienced 0.004 microT. Cellular transcript levels were assessed using slot blots and quantified using a Phosphorimager. Blots were hybridised using probes against HSP 70a, Histone 1.9, and Copia. The low frequency EMFs very significantly decreased transcript levels, indicating that experimental responses may be influenced by previous exposure or lack of previous exposure.

摘要

在内部场强为0.004微特斯拉的微型金属盒中饲养的果蝇幼虫,用50赫兹、8微特斯拉的磁场处理20分钟。对照组经历0.004微特斯拉。使用狭缝印迹法评估细胞转录水平,并使用磷光成像仪进行定量。印迹用针对热休克蛋白70a、组蛋白1.9和考皮亚的探针进行杂交。低频电磁场非常显著地降低了转录水平,表明实验反应可能受到先前暴露或缺乏先前暴露的影响。

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