Hodges K B, Vnencak-Jones C L, Larson R S, Kinney M C
Department of Pathology, Vanderbilt University Medical Center, Nashville, TN, USA.
Hum Pathol. 1999 Feb;30(2):173-7. doi: 10.1016/s0046-8177(99)90272-1.
Microsatellite instability (MSI) is a recently described type of genetic alteration resulting from defects in the DNA mismatch repair genes that appears to play an integral role in neoplastic transformation. MSI has been described in a wide variety of malignancies; however, data regarding the role of MSI in the pathogenesis of non-Hodgkin's lymphoma (NHL) are limited. MSI appears to be important in some T-cell lymphomas, including ALCL arising in immunocompromised patients. In addition, MSI has recently been identified in CD30+ cutaneous lymphoproliferative processes and lymphoblastic lymphoma. In this study, we have analyzed five well-characterized cases of systemic T-cell ALCL arising in immunocompetent patients for the presence of MSI. Genomic DNA isolated from paired normal and tumor tissue was analyzed at seven microsatellite loci by polymerase chain reaction. We were unable to identify MSI or loss of heterozygosity (LOH) in our cases, suggesting that abnormalities in the DNA mismatch repair system do not play a major role in the pathogenesis of most systemic ALCL. Our data provide additional molecular evidence that the various subgroups of lymphoma with ALCL morphology are biologically distinct processes.
微卫星不稳定性（MSI）是一种最近被描述的基因改变类型，由DNA错配修复基因缺陷导致，似乎在肿瘤转化中起重要作用。MSI已在多种恶性肿瘤中被描述；然而，关于MSI在非霍奇金淋巴瘤（NHL）发病机制中的作用的数据有限。MSI在一些T细胞淋巴瘤中似乎很重要，包括免疫功能低下患者发生的间变性大细胞淋巴瘤（ALCL）。此外，MSI最近在CD30+皮肤淋巴细胞增生性病变和淋巴细胞性淋巴瘤中被发现。在本研究中，我们分析了5例免疫功能正常患者发生的系统性T细胞ALCL的典型病例，以检测MSI的存在。通过聚合酶链反应在7个微卫星位点分析从配对的正常组织和肿瘤组织中分离的基因组DNA。我们在病例中未能识别出MSI或杂合性缺失（LOH），这表明DNA错配修复系统异常在大多数系统性ALCL的发病机制中不发挥主要作用。我们的数据提供了额外的分子证据，表明具有ALCL形态的淋巴瘤的各个亚组是生物学上不同的过程。
