Gamberi B, Gaidano G, Parsa N, Carbone A, Roncella S, Knowles D M, Louie D C, Shibata D, Chaganti R S, Dalla-Favera R
Department of Pathology, College of Physicians and Surgeons, Columbia University, New York 10032, USA.
Blood. 1997 Feb 1;89(3):975-9.
Microsatellite instability (MSI), a symptom of defect in DNA mismatch repair function, represents a type of genomic instability frequently detected in many types of cancers. However, the involvement of MSI in non-Hodgkin's lymphomas (NHL) has not been conclusively investigated. In this study, we have tested the presence of MSI in 69 cases of B-cell NHL (B-NHL) representative of the various histologic categories of the disease and including 17 cases of acquired immunodeficiency syndrome (AIDS)-related B-NHL (AIDS-NHL). In addition, for selected B-NHL cases, consecutive samples obtained before and after clinical progression (with and without concomitant histologic transformation) were also investigated. Five distinct microsatellite repeats (2 dinucleotide, 2 trinucleotide, and 1 tetranucleotide repeats) were analyzed by polymerase chain reaction in all cases. MSI, defined by the presence of microsatellite alterations in two or more of the five microsatellite loci tested, was not found in NHL. In contrast to a previous study reporting the frequent association between MSI and AIDS-NHL, we found this abnormality in only 1 of 17 cases of AIDS-NHL representative of the major subtypes. Overall, these data indicate that defects in DNA mismatch repair do not contribute significantly to the molecular pathogenesis of B-NHL.
微卫星不稳定性（MSI）是DNA错配修复功能缺陷的一种表现，代表了一种在多种癌症中经常检测到的基因组不稳定性类型。然而，MSI在非霍奇金淋巴瘤（NHL）中的作用尚未得到确凿研究。在本研究中，我们检测了69例B细胞NHL（B-NHL）中MSI的存在情况，这些病例代表了该疾病的各种组织学类别，包括17例获得性免疫缺陷综合征（AIDS）相关的B-NHL（AIDS-NHL）。此外，对于选定的B-NHL病例，还研究了临床进展前后（伴有或不伴有组织学转化）获得的连续样本。在所有病例中，通过聚合酶链反应分析了五个不同的微卫星重复序列（2个二核苷酸、2个三核苷酸和1个四核苷酸重复序列）。MSI定义为在所检测的五个微卫星位点中的两个或更多位点存在微卫星改变，在NHL中未发现。与先前报道MSI与AIDS-NHL频繁相关的研究相反，我们在17例代表主要亚型的AIDS-NHL病例中仅发现1例存在这种异常。总体而言，这些数据表明DNA错配修复缺陷对B-NHL的分子发病机制没有显著贡献。
