Potts R J, Newbury C J, Smith G, Notarianni L J, Jefferies T M
School of Pharmacy and Pharmacology, University of Bath, Bath, BA2 7AY, UK.
Mutat Res. 1999 Jan 25;423(1-2):103-11. doi: 10.1016/s0027-5107(98)00242-5.
Cigarette smoke is a rich source of mutagens and carcinogens; thus, we have investigated the effects of male smoking on the DNA of human sperm. This was performed using the sperm chromatin structure assay (SCSA), which measures the sensitivity of sperm DNA to acid induced denaturation, and the terminal deoxynucleotidyl transferase assay (TdTA), which measures DNA strand breaks by addition of the biotinylated nucleotide dUTP to 3'-OH ends of DNA, sites of DNA breakage, using the enzyme terminal deoxynucleotidyl transferase. Sperm from subjects who smoked were significantly more sensitive to acid induced denaturation than non-smokers (P<0.02) and possessed higher levels of DNA strand breaks (P<0.05). We hypothesise that smoking damages the chromatin structure and produces endogenous DNA strand breaks in human sperm. These changes may result in sperm DNA mutations, that predispose offspring to greater risk of malformations, cancer and genetic diseases.
香烟烟雾是诱变剂和致癌物质的丰富来源；因此，我们研究了男性吸烟对人类精子DNA的影响。这是通过精子染色质结构分析（SCSA）来进行的，该分析测量精子DNA对酸诱导变性的敏感性，以及末端脱氧核苷酸转移酶分析（TdTA），该分析通过将生物素化的核苷酸dUTP添加到DNA的3'-OH末端来测量DNA链断裂，即使用末端脱氧核苷酸转移酶来确定DNA断裂位点。吸烟者的精子对酸诱导变性的敏感性明显高于不吸烟者（P < 0.02），并且具有更高水平的DNA链断裂（P < 0.05）。我们推测吸烟会破坏染色质结构并在人类精子中产生内源性DNA链断裂。这些变化可能导致精子DNA突变，使后代面临更高的畸形、癌症和遗传疾病风险。
