Weijtens M J, Reinders R D, Urlings H A, Van der Plas J
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
J Appl Microbiol. 1999 Jan;86(1):63-70. doi: 10.1046/j.1365-2672.1999.00636.x.
The excretion of campylobacter by eight individually housed fattening pigs was monitored during 15 weeks. Rectal faeces samples were collected six times from these pigs and twice from their mothers (seven sows). Campylobacter was cultured from these samples on Preston medium. In some pigs, samples positive for campylobacter alternated with negative samples. Campylobacter was detected in at least four of the six samples collected per fattening pig. The average campylobacter count per sampling showed a decreasing trend (P < 0.001). Of the seven sows, six were shown to excrete campylobacter. Campylobacter isolates of pigs and sows were typed using the Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR); 28 different campylobacter types were distinguished. Up to five different types were isolated from single faeces samples. Individual porkers could harbour up to eight types during their fattening period. The three types most frequently isolated from the fattening pigs were also present in the sows.
在15周内对8头单独饲养的育肥猪的弯曲杆菌排泄情况进行了监测。从这些猪身上采集了6次直肠粪便样本，从它们的母亲（7头母猪）身上采集了2次样本。弯曲杆菌在普雷斯顿培养基上从这些样本中培养出来。在一些猪中，弯曲杆菌阳性样本与阴性样本交替出现。每头育肥猪采集的6个样本中至少有4个检测到弯曲杆菌。每次采样的弯曲杆菌平均计数呈下降趋势（P<0.001）。7头母猪中有6头被证明排泄弯曲杆菌。使用肠杆菌重复基因间共有序列聚合酶链反应（ERIC-PCR）对猪和母猪的弯曲杆菌分离株进行分型；区分出28种不同的弯曲杆菌类型。单个粪便样本中最多可分离出5种不同类型。个体育肥猪在育肥期可能携带多达8种类型。从育肥猪中最常分离出的三种类型在母猪中也存在。
