Anses, Laboratoire de Ploufragan-Plouzané, unité Hygiène et Qualité des Produits Avicoles et Porcins, BP53, 22440 Ploufragan, France.
Vet Microbiol. 2011 Dec 29;154(1-2):163-70. doi: 10.1016/j.vetmic.2011.07.001. Epub 2011 Jul 7.
Sows have been identified as a source of Campylobacter contamination in piglets. We carried out a one-year study, in 2008, at 53 farrow-to-finish farms in Brittany, France, to determine the proportion of sows excreting Campylobacter. We also determined the genotypes of the Campylobacter isolates. Moreover, Generalized Estimating Equations including repeated effects were used to assess the association between management practices and farm characteristics, and risk of Campylobacter shedding by sows. Per farm, 10 feces samples from sows were collected from selected sites (maternity, service area, gestation area) on the farms. Campylobacter isolates were identified by PCR and typed by PFGE. Campylobacter was detected in 25.1% of the 530 samples from sows, and 67% of the 53 pig farms had at least one positive sample (of 10 taken). All the Campylobacter isolates belonged to the Campylobacter coli species. They displayed a very high level of genetic diversity, also inside farms and few genotypes were common to several farms. Warmer months, large farms, and individual housing for sows were identified as risk indicators of Campylobacter shedding by sows. A short delay between sampling and treatment of the samples should be considered, to improve the detection of the bacterium in the feces samples.
母猪已被确定为仔猪中弯曲杆菌污染的来源。我们在 2008 年对法国布列塔尼的 53 个分娩至育肥农场进行了为期一年的研究，以确定排泄弯曲杆菌的母猪比例。我们还确定了弯曲杆菌分离株的基因型。此外，使用广义估计方程（包括重复效应）来评估管理实践和农场特征与母猪弯曲杆菌脱落之间的关联。每 10 个农场，从母猪的选定地点（产房、服务区、妊娠区）采集 10 个粪便样本。通过 PCR 鉴定弯曲杆菌分离株，并通过 PFGE 对其进行分型。在 530 份母猪粪便样本中，有 25.1%检测到弯曲杆菌，在 53 个养猪场中，有 67%至少有一个阳性样本（采集了 10 个样本）。所有弯曲杆菌分离株均属于弯曲杆菌 coli 种。它们表现出非常高的遗传多样性，即使在农场内部，也很少有基因型在多个农场中共同存在。温暖的月份、大型农场和母猪的单独饲养被确定为母猪弯曲杆菌脱落的风险指标。应考虑在采样和处理样本之间进行短暂延迟，以提高粪便样本中细菌的检测率。
