Weijtens M J, van der Plas J, Bijker P G, Urlings H A, Koster D, van Logtestijn J G, Huis in't Veld J H
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
J Appl Microbiol. 1997 Dec;83(6):693-8. doi: 10.1046/j.1365-2672.1997.00301.x.
The campylobacter infection of 10 sows and their piglets was monitored. These pigs were kept on two multiplier farms. Rectal faeces samples were taken from the sows shortly before littering and at different intervals after littering. Swab samples of rectal content were taken from six piglets per sow at different intervals after birth. Nine sows were shown to be infected with campylobacter before litter and all sows after litter, with an average colony count of 4.1 in log N g-1 of faeces. Half of the piglets became infected with campylobacter during the first week of life and 85%, after four weeks. Two genetic subtyping methods (ERIC-PCR and RFLP) were used to study the relationships between campylobacter isolates from sows and piglets. A large diversity of campylobacter subtypes was found. Nevertheless, piglets and their mothers often harboured campylobacter isolates with identical genetic subtyping profiles, suggesting that piglets become infected via their mothers. However, observed similarities in genetic subtyping profiles between campylobacters isolated on different farms made this difficult to prove.
对10头母猪及其仔猪的弯曲杆菌感染情况进行了监测。这些猪饲养在两个扩繁场。在母猪产仔前不久以及产仔后的不同时间段采集直肠粪便样本。在每头母猪的仔猪出生后的不同时间段，从每头母猪的6头仔猪中采集直肠内容物拭子样本。结果显示，9头母猪在产仔前感染了弯曲杆菌，所有母猪在产仔后均感染，粪便中弯曲杆菌的平均菌落数为每克4.1（以对数N计）。一半的仔猪在出生后的第一周感染了弯曲杆菌，四周后感染率达到85%。使用两种基因分型方法（ERIC-PCR和RFLP）研究母猪和仔猪分离出的弯曲杆菌之间的关系。发现了多种弯曲杆菌亚型。然而，仔猪及其母亲通常携带基因分型谱相同的弯曲杆菌分离株，这表明仔猪是通过其母亲感染的。然而，在不同农场分离出的弯曲杆菌之间观察到的基因分型谱相似性使得这一点难以证实。
