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N-乙基马来酰亚胺(NEM)刺激的骆驼红细胞被动钾转运:年龄、呋塞米、氟化钠和冈田酸的抑制作用。

N-ethylmaliemide (NEM)-stimulated passive potassium transport in camel erythrocytes: inhibitory effects of age, furosemide, sodium fluoride and okadaic acid.

作者信息

Gharaibeh N S

机构信息

Department of Physiology & Biochemistry, Jordan University of Science and Technology, Irbid, Jordan.

出版信息

Vet J. 1999 Jan;157(1):57-60. doi: 10.1053/tvjl.1998.0245.

DOI:10.1053/tvjl.1998.0245
PMID:10030129
Abstract

The effects of age, and the inhibitory effects of furosemide, sodium fluoride, and okadaic acid on N-ethylmaliemide (NEM)-stimulated, ouabain-resistant potassium influx were investigated in camel erythrocytes. The NEM-stimulated influx which declined with the age of erythrocytes, was partially inhibited by 2 mmol/L furosemide, 20 mmol/L sodium fluoride, and completely inhibited by 150 nM okadaic acid. The effect of all chemical inhibitors was significant if added before the NEM pretreatment.

摘要

在骆驼红细胞中研究了年龄的影响以及呋塞米、氟化钠和冈田酸对N - 乙基马来酰亚胺(NEM)刺激的哇巴因抗性钾内流的抑制作用。NEM刺激的内流随红细胞年龄增加而下降,2 mmol/L呋塞米、20 mmol/L氟化钠可部分抑制该内流,150 nM冈田酸可完全抑制。若在NEM预处理前添加所有化学抑制剂,其作用均显著。

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