Gharaibeh N S, Rawashdeh N M
Department of Physiology and Biochemistry, Jordan University of Science and Technology, Irbid.
Membr Biochem. 1993 Jul-Sep;10(3):181-7. doi: 10.3109/09687689309150265.
The inhibitory effects of furosemide, sodium fluoride, and age on volume-dependent, ouabain-resistant K+ influx were investigated in camel red blood cells. Swelling of young camel erythrocytes hypotonically stimulates ouabain-resistant potassium influx, a response that was lacking in old camel erythrocytes. The swelling-stimulated influx was partially inhibited by 1 mM furosemide and by 10 and 20 mM sodium fluoride. The inhibitory effect of furosemide was significantly increased if rubidium was added to the flux media. There was a significant correlation between potassium influx in normo- and hypotonic media which might indicate that the anion-dependent transport system operates, to some extent, to regulate cell volume.
在骆驼红细胞中研究了呋塞米、氟化钠和年龄对容量依赖性哇巴因抗性钾内流的抑制作用。低渗条件下年轻骆驼红细胞肿胀可刺激哇巴因抗性钾内流，而老年骆驼红细胞缺乏这种反应。肿胀刺激的内流部分受到1 mM呋塞米以及10 mM和20 mM氟化钠的抑制。如果在通量介质中加入铷，呋塞米的抑制作用会显著增强。等渗和低渗介质中的钾内流之间存在显著相关性，这可能表明阴离子依赖性转运系统在一定程度上参与调节细胞体积。
