Jacobs B B, Leonard L, Parks R C
J Natl Cancer Inst. 1976 Aug;57(2):345-8. doi: 10.1093/jnci/57.2.345.
Allotransplantable lines of the BALB/c (H-2d) Leydig cell tumor C4092 were established in DBA/1 (H-2q) mice after maintenance in organ culture. These modified tumors had reduced immunogenicity but were recognized and rejected by previously sensitized DBA/1 mice. The addition of 3.4 X 10(3)-1 X 10(5) peritoneal exudate cells (PEC) from untreated BALB/c mice to cell suspensions of the modified tumor did not restore Immunogenicity when the cell mixture was Inoculated sc Into DBA/1 recipients. However, when equivalent numbers of PEC or spleen cells were inoculated iv into animals receiving the tumor cell suspension sc, the acceptance of the tumors was significantly reduced or prevented. This indicated that the BALB/c lymphoid cells inoculated iv stimulated the immune system of the host, which in turn recognized the tumor cell inoculum. The lack of effect of the BALB/c lymphoid cells when admixed with the tumor did not support the assumption that the loss of passenger lymphoid cells was responsible for the allotransplantability of grafts after organ culture explantation. Additional evidence was presented that supports our view that the reduced immunogenicity was associated with a phenotypic modification of the tumor cells per se.
BALB/c（H-2d）睾丸间质细胞瘤C4092的同种移植系在器官培养中维持后，在DBA/1（H-2q）小鼠中建立。这些经过修饰的肿瘤免疫原性降低，但被预先致敏的DBA/1小鼠识别并排斥。当将来自未处理的BALB/c小鼠的3.4×10³ - 1×10⁵个腹腔渗出细胞（PEC）添加到修饰肿瘤的细胞悬液中，并将细胞混合物皮下接种到DBA/1受体小鼠时，免疫原性并未恢复。然而，当将等量的PEC或脾细胞静脉内接种到接受皮下肿瘤细胞悬液的动物中时，肿瘤的接受率显著降低或被阻止。这表明静脉内接种的BALB/c淋巴细胞刺激了宿主的免疫系统，进而识别了肿瘤细胞接种物。当BALB/c淋巴细胞与肿瘤混合时缺乏作用，这并不支持以下假设，即过客淋巴细胞的丧失是器官培养外植后移植物同种移植性的原因。还提供了额外的证据支持我们的观点，即免疫原性降低与肿瘤细胞本身的表型改变有关。
