Tazawa Y, Ishikawa S, Furuhata Y, Kikuchi Y, Okada J
Clinical Laboratory, NTT Kanto Teishin Hospital, 5-9-22 Higashigotanda, Shinagawa-ku, Tokyo 141-8625, Japan.
Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi. 1998;9(2):59-65.
Recently, an automatic instrument for culturing acid-fast bacteria in liquid media has been introduced in Japan to accelerate and increase the detection of the bacteria as well as the work e ciency of test lab technicians. We compared the MGIT (Mycobacterium Growth Indicator Tube) method and the two conventional culture methods used in our lab by testing 176 specimens (130 cases) submitted for acid-fast testing between July 27 and September 18, 1998. BACTEC MGIT 960 (Nippon Becton Dickinson) was used in the MGIT method while Ogawa K medium and Kudo PD medium were used for our conventional culture methods. Of the 176 specimens, 26 specimens were MGIT positive, 22 specimens were Ogawa K medium positive, and 21 specimens were Kudo PD medium positive. Among these three culture methods, M. tuberculosis was found in 8 specimens, M. avium was found in 9 specimens, M. intracellulare was found in 4 specimens, and other acid-fast bacteria were found in 5 specimens. It took an average of 13.5 days to detect M. tuberculosis by the MGIT method, 23.0 days by the Ogawa K medium, and 24.3 days by the Kudo PD medium, suggesting that the MGIT method is signi cantly faster than the conventional methods (p < 0.05). The MGIT method took an average of 5.7 days to detect M. avium and M. intracellulare while the two conventional methods required more than 20 days, suggesting that the MGIT method is signi cantly faster than the conventional methods (p < 0.05). The BACTEC MGIT 960 was extremely useful for clinical diagnosis in some of the cases and our results indicated that the MGIT method led to more rapid diagnosis and earlier treatment than the conventional methods.
最近，日本推出了一种用于在液体培养基中培养抗酸菌的自动仪器，以加速并增加对该类细菌的检测，同时提高检验实验室技术人员的工作效率。我们通过检测1998年7月27日至9月18日期间提交的176份（130例）用于抗酸检测的标本，比较了MGIT（分枝杆菌生长指示管）法与我们实验室使用的两种传统培养方法。MGIT法使用BACTEC MGIT 960（日本BD公司），而我们的传统培养方法使用小川K培养基和工藤PD培养基。在这176份标本中，26份标本MGIT法检测呈阳性，22份标本小川K培养基检测呈阳性，21份标本工藤PD培养基检测呈阳性。在这三种培养方法中，8份标本检测出结核分枝杆菌，9份标本检测出鸟分枝杆菌，4份标本检测出胞内分枝杆菌，5份标本检测出其他抗酸菌。MGIT法检测结核分枝杆菌平均需要13.5天，小川K培养基法需要23.0天，工藤PD培养基法需要24.3天，这表明MGIT法明显比传统方法更快（p<0.05）。MGIT法检测鸟分枝杆菌和胞内分枝杆菌平均需要5.7天，而两种传统方法需要20多天，这表明MGIT法明显比传统方法更快（p<0.05）。BACTEC MGIT 960在某些病例的临床诊断中非常有用，我们的结果表明，MGIT法比传统方法能实现更快速的诊断和更早的治疗。
