Saito H, Kashiwabara Y, Sato K, Katayama T, Kwon H H, Tomioka H
National Institute for Leprosy Research, Tokyo, Japan.
Kekkaku. 1996 Jun;71(6):399-405.
The BBL MGIT Mycobacteria Growth Indicator Tube is a novel broth based culture system for the detection of mycobacteria from clinical specimens. The tubes consist of a fluorescent indicator embedded in silicone on the bottom of a 16x100 mm round-bottom tube, filled with 4ml of an enriched BBL Middlebrook 7H9 broth base, with 0.25% glycerol. Actively growing mycobacteria consume the oxygen dissolved in the medium and fluorescence will occur when the tubes are observed with a 365nm transilluminator. The purpose of this study is to evaluate comparatively MGIT with 1% Ogawa egg medium by using two hundred and forty-five clinical specimens. The samples were digested, decontaminated and concentrated for culture using N-acetyl-L-cysteine-sodium hydroxide method. Fifty-nine of 245 (24%) clinical samples were culture positive for mycobacteria (43 M. tuberculosis complex, 12 M. avium complex and 4 other species) by one or both test systems. The MGIT detected 4 isolates of M. tuberculosis complex and 6 isolates of M. avium complex not recovered by the Ogawa egg medium, respectively. The mean time of detection of M. tuberculosis complex was 13 days (range: 2-26 days) and 19 days (range: 8-31 days) for MGIT and Ogawa egg medium, respectively, and that of M. avium complex was 5 days (range: 2-8 days) and 16 days (range: 6-22 days) for the MGIT and Ogawa egg medium, respectively. Overall, the MGIT is a sensitive culture system for the detection of mycobacteria from clinical specimens, is easy to use and may be applicable to clinical laboratories.
BBL MGIT分枝杆菌生长指示管是一种新型的基于肉汤的培养系统，用于从临床标本中检测分枝杆菌。这些管子由嵌入在16×100毫米圆底管底部硅胶中的荧光指示剂组成，管内装有4毫升添加了0.25%甘油的改良BBL米氏7H9肉汤基础培养基。活跃生长的分枝杆菌消耗培养基中溶解的氧气，当用365纳米透照仪观察管子时会产生荧光。本研究的目的是通过使用245份临床标本，对MGIT与1%小川鸡蛋培养基进行比较评估。使用N-乙酰-L-半胱氨酸-氢氧化钠法对样本进行消化、去污和浓缩以进行培养。245份临床样本中有59份（24%）通过一种或两种检测系统培养出分枝杆菌阳性（43份结核分枝杆菌复合群、12份鸟分枝杆菌复合群和4份其他菌种）。MGIT分别检测出4份结核分枝杆菌复合群菌株和6份鸟分枝杆菌复合群菌株，而小川鸡蛋培养基未培养出这些菌株。结核分枝杆菌复合群的平均检测时间，MGIT为13天（范围：2 - 26天），小川鸡蛋培养基为19天（范围：8 - 31天）；鸟分枝杆菌复合群的平均检测时间，MGIT为5天（范围：2 - 8天），小川鸡蛋培养基为16天（范围：6 - 22天）。总体而言，MGIT是一种用于从临床标本中检测分枝杆菌的敏感培养系统，使用方便，可能适用于临床实验室。
