Flavell A J, Knox M R, Pearce S R, Ellis T H
Department of Biochemistry, University of Dundee, UK.
Plant J. 1998 Dec;16(5):643-50. doi: 10.1046/j.1365-313x.1998.00334.x.
Two assays based upon PCR detection of a polymorphic PDR1 retrotransposon insertion in Pisum sativum have been developed. Both methods involve PCR with primers derived from the transposon and flanking DNA. The first method uses a dot assay for PCR product detection which could be fully automated for handling thousands of samples. The second method, which is designed to handle lower numbers, requires a single PCR and gel lane per sample. Both methods yield co-dominant markers, with presence and absence of the transposon insertion independently scorable, and both could in principle be applied to any transposable element in any plant species.
已开发出两种基于PCR检测豌豆中多态性PDR1逆转座子插入的检测方法。这两种方法都涉及使用源自转座子和侧翼DNA的引物进行PCR。第一种方法使用斑点检测法来检测PCR产物，该方法可完全自动化以处理数千个样本。第二种方法旨在处理数量较少的样本，每个样本需要一次PCR和一个凝胶泳道。两种方法都产生共显性标记，转座子插入的存在与否可独立评分，并且原则上两种方法都可应用于任何植物物种中的任何转座元件。
