Bechert T, Heck S, Fleig U, Diekmann S, Hegemann J H
Abteilung Molekularbiologie, Institut für Molekulare Biotechnologie, Postfach 100813, 07708 Jena, Germany and Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, Frankfurter Strasse 107,35392 Giessen.
Nucleic Acids Res. 1999 Mar 15;27(6):1444-9. doi: 10.1093/nar/27.6.1444.
All 16 centromere DNA regions of Saccharomyces cerevisiae including 90 bp framing sequences on either side were cloned. These 300 bp long centromere regions were analysed by native polyacrylamide gel electrophoresis and found to display a reduced mobility indicative of DNA curvature. The degree of curvature is centromere dependent. The experimental data were confirmed by computer analysis of the 3-dimensional structure of the CEN DNAs. Altogether these data provide further evidence for a model for budding yeast centromeres in which CEN DNA structure could be important for the assembly, activity and/or regulation of the centromere protein-DNA complex.
酿酒酵母的所有16个着丝粒DNA区域，包括两侧90bp的框架序列，都被克隆出来。对这些300bp长的着丝粒区域进行了非变性聚丙烯酰胺凝胶电泳分析，发现其迁移率降低，表明存在DNA弯曲。弯曲程度取决于着丝粒。通过对CEN DNA三维结构的计算机分析，证实了实验数据。这些数据共同为芽殖酵母着丝粒模型提供了进一步的证据，其中CEN DNA结构对着丝粒蛋白-DNA复合物的组装、活性和/或调节可能很重要。
