Zeng H, Chittur K K, Lacefield W R
Materials Science, University of Alabama at Birmingham, 35294, USA.
Biomaterials. 1999 Feb;20(4):377-84. doi: 10.1016/s0142-9612(98)00184-7.
The protein adsorption behavior of thin films of calcium phosphate (CaP) bioceramic and titanium (Ti) was studied in this research. The thin films were produced with an ion beam sputter deposition technique using targets of hydroxyapatite (HA), fluorapatite (FA) and titanium (Ti). Fourier transform infrared spectroscopy (FTIR) with attenuated total internal reflectance (ATR) was used to evaluate protein adsorption on these surfaces. This study showed that surface composition and structure influenced the kinetics of protein adsorption and the structure of adsorbed protein. CaP surfaces adsorbed greater amount of protein than the Ti surface, and caused more alteration of the structure of adsorbed BSA than did the Ti surface. The differences in protein adsorption behavior could result in very different initial cellular behavior on CaP and Ti implant surfaces.
本研究考察了磷酸钙（CaP）生物陶瓷薄膜和钛（Ti）的蛋白质吸附行为。采用离子束溅射沉积技术，以羟基磷灰石（HA）、氟磷灰石（FA）和钛（Ti）为靶材制备薄膜。利用衰减全反射傅里叶变换红外光谱（FTIR-ATR）评估这些表面上的蛋白质吸附情况。该研究表明，表面组成和结构影响蛋白质吸附动力学以及吸附蛋白质的结构。CaP表面比Ti表面吸附更多的蛋白质，并且与Ti表面相比，其引起的吸附牛血清白蛋白（BSA）结构变化更大。蛋白质吸附行为的差异可能导致在CaP和Ti植入物表面上细胞的初始行为截然不同。
