Stenport Victoria F, Olander Julia, Kjellin Per, Currie Fredrik, Sul Young-Taeg, Anna Arvidsson
Department of Prosthetic Dentistry/Dental Materials Science, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Göteborg Sweden.
Promimic AB, Göteborg Sweden.
J Oral Maxillofac Res. 2015 Dec 31;6(4):e1. doi: 10.5037/jomr.2015.6401. eCollection 2015 Oct-Dec.
To compare the properties of calcium phosphate precipitation on four different bioactive surface preparations and one control surface in the simulated body fluid model with added collagen type I.
Blasted titanium discs were treated with four different surface modifications, alkali and heat, sodium fluoride, anodic oxidation and hydroxyapatite coating. The discs were divided into five groups where one group, the blasted, served as control. The discs were immersed in simulated body fluid and collagen for 24 h, 3 days, 1 week and 2 weeks and then analysed by optical interferometry, scanning electron microscopy/energy dispersive X-ray analysis and X-ray photoelectron spectroscopy.
All surfaces show small precipitates after 3 days which with longer immersion times increase. After 2 weeks the surfaces were completely covered with precipitates, and Ca/P ratios were approximately 1.3, independently on surface preparation. The fluoridated discs showed significantly (P ≤ 0.05) higher degree of CaP after one week of immersion as compared to the other surface preparations. The collagen type I content increased with time, as reflected by increased nitrogen content.
The results from this study indicate that a fluoridated titanium surface may favour precipitation of calcium phosphate in the presence of collagen type I, as compared to the other surface treatments of the present study.
在添加了I型胶原蛋白的模拟体液模型中，比较四种不同生物活性表面处理以及一种对照表面上磷酸钙沉淀的特性。
对喷砂处理的钛盘进行四种不同的表面改性处理，即碱热处理、氟化钠处理、阳极氧化处理和羟基磷灰石涂层处理。将这些钛盘分为五组，其中一组喷砂处理的作为对照组。将钛盘浸入模拟体液和胶原蛋白中24小时、3天、1周和2周，然后通过光学干涉测量法、扫描电子显微镜/能量色散X射线分析和X射线光电子能谱进行分析。
3天后所有表面均出现小的沉淀物，随着浸泡时间延长沉淀物增加。2周后，表面完全被沉淀物覆盖，且钙磷比约为1.3，与表面处理方式无关。浸泡1周后，氟化处理的钛盘与其他表面处理相比，磷酸钙的沉积程度显著更高（P≤0.05）。I型胶原蛋白含量随时间增加，这可通过氮含量增加得以体现。
本研究结果表明，与本研究中的其他表面处理相比，氟化钛表面在存在I型胶原蛋白的情况下可能更有利于磷酸钙沉淀。
