Dumanian G A, McClinton M A, Brushart T M
Division of Plastic and Reconstructive Surgery, Northwestern University Medical School, Chicago, IL, USA.
J Hand Surg Am. 1999 Jan;24(1):30-6. doi: 10.1053/jhsu.1999.jhsu24a0030.
We developed a new quantitative rat sciatic nerve model to test whether free fat grafts can reduce postoperative perineural scar formation. Epineurectomies of sciatic nerves were performed to create scar. The force required to distract the nerve a unit distance was measured after surgery to determine the time of maximal scar formation. Nerve stiffness normalized for rat weight was statistically greater at 2 months after the initial dissection (0.097+/-0.009 g/mm/g rat weight; n = 10 limbs) than rat limbs that had not undergone a previous dissection (0.075+/-0.012 g/mm/g rat weight). Perineural scar thickness was thicker at 2 months than the perineural tissue in preoperative controls. Free fat grafts decreased nerve stiffness at 2 months (0.078+/-0.012 g/mm/g rat weight) in comparison to the contralateral surgical control limb without a fat graft (0.094+/-0.014 g/mm/g rat weight). Free fat grafts reduced the strength of postoperative perineural scar in this surgical model; however, they were associated with an unexpected finding of substantial postoperative neuropathy.
我们开发了一种新的定量大鼠坐骨神经模型，以测试游离脂肪移植是否能减少术后神经周围瘢痕形成。对坐骨神经进行神经外膜切除术以形成瘢痕。术后测量将神经牵拉单位距离所需的力，以确定瘢痕形成最大的时间。在初次解剖后2个月，经大鼠体重标准化的神经硬度在统计学上高于未进行过先前解剖的大鼠肢体（0.097±0.009 g/mm/g大鼠体重；n = 10只肢体）（0.075±0.012 g/mm/g大鼠体重）。2个月时神经周围瘢痕厚度比术前对照组的神经周围组织更厚。与未进行脂肪移植的对侧手术对照肢体（0.094±0.014 g/mm/g大鼠体重）相比，游离脂肪移植在2个月时降低了神经硬度（0.078±0.012 g/mm/g大鼠体重）。在这个手术模型中，游离脂肪移植降低了术后神经周围瘢痕的强度；然而，它们与术后出现大量神经病变这一意外发现有关。
