在特应性遗传易感性模型中对即刻皮肤超敏反应的定量评估。

Quantitative assessment of immediate cutaneous hypersensitivity in a model of genetic predisposition to atopy.

作者信息

Daser A, Bätjer N, Kölsch U, Kötz K, Schmeling H, Schou C, Renz H

机构信息

Institute of Clinical Chemistry and Biochemistry, Virchow Klinics of the Humboldt University, Berlin, Germany.

出版信息

Int Arch Allergy Immunol. 1998 Dec;117(4):239-43. doi: 10.1159/000024017.

DOI:10.1159/000024017

PMID:10048895

Abstract

Genetic predisposition and environmental factors modulate the expression of allergic phenotypes. The frequent allergic phenotype 'immediate cutaneous hypersensitivity' was established in mice as a model for atopy. Genetic dissection of this trait requires a robust procedure to assess the allergic phenotype. To this end, different mouse strains were immunized with birch pollen extract. Immediate cutaneous hypersensitivity reactions were induced through intradermal allergen exposure. Wheel formation was quantitated and expressed as a hypersensitivity score according to the bonitur method. This procedure identified A/J and C57BL/6 mice as high- and low-responder strains, respectively. Crosses of A/J and C57BL/6 mice should allow the characterization of mendelian factors responsible for the two extreme phenotypes identified here.

摘要

遗传易感性和环境因素调节过敏表型的表达。常见的过敏表型“即刻皮肤超敏反应”在小鼠中被确立为特应性疾病的模型。对这一性状进行遗传剖析需要一个可靠的程序来评估过敏表型。为此，用桦树花粉提取物对不同的小鼠品系进行免疫。通过皮内接触变应原诱导即刻皮肤超敏反应。根据bonitur方法对风团形成进行定量并表示为超敏评分。该程序确定A/J和C57BL/6小鼠分别为高反应和低反应品系。A/J和C57BL/6小鼠的杂交应该能够鉴定出导致此处所确定的两种极端表型的孟德尔因子。