Prows Daniel R, Horner Michelle L
Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH 45267-0056, USA.
Mamm Genome. 2002 Mar;13(3):127-33. doi: 10.1007/BF02684016.
Recombinant inbred (RI) strains are a valuable tool in mouse genetics to rapidly map the location of a new locus. Because RI strains have been typed for hundreds of genetic markers, the genotypes of individual strains within an RI set can be examined to identify specific strain(s) containing the desired region(s) of interest (e.g., one or more quantitative trait loci, QTLs) for subsequent phenotype testing. Specific RI strains might also be identified for use as progenitors in the construction of consomic (chromosome substitution strains or CSSs) or congenic lines or for use in the RI strain test (RIST). To quickly identify the genetic contributions of the parental A/J (A) and C57BL/6J (B) strains, we have generated chromosome maps for each commercially available AXB and BXA RI strain, in which the genetic loci are color-coded to signify the parent of origin. To further assist in strain selection for further breeding schemes, the percentages of A and B parental contributions were calculated, based on the total number of typed markers in the database for each strain. With these data, one can rapidly select the RI strain(s) carrying the desired donor and recipient strain region(s). Because points of recombination are known, starting with RI mice to generate CSSs or congenic lines immediately reduces genomewide screening to those donor-strain regions not already homozygous in the recipient strain. Two examples are presented to demonstrate potential uses of the generated chromosome maps: to select RI strains to construct congenic lines and to perform an RIST for Aliq1, a QTL linked to ozone-induced acute lung injury survival.
重组近交（RI）系是小鼠遗传学中用于快速定位新基因座位置的宝贵工具。由于RI系已针对数百个遗传标记进行了分型，因此可以检查RI组内各个品系的基因型，以鉴定包含感兴趣的特定区域（例如，一个或多个数量性状基因座，QTL）的特定品系，用于后续的表型测试。还可以鉴定特定的RI品系，用作构建染色体代换系（CSS）或近交系的祖系，或用于RI系测试（RIST）。为了快速确定亲本A/J（A）和C57BL/6J（B）品系的遗传贡献，我们为每个市售的AXB和BXA RI品系生成了染色体图谱，其中遗传位点用颜色编码以表示起源亲本。为了进一步协助选择用于进一步育种方案的品系，根据每个品系数据库中已分型标记的总数计算A和B亲本贡献的百分比。利用这些数据，可以快速选择携带所需供体和受体品系区域的RI品系。由于重组点是已知的，从RI小鼠开始生成CSS或近交系可立即将全基因组筛选减少到受体品系中尚未纯合的那些供体品系区域。给出了两个例子来说明所生成的染色体图谱的潜在用途：选择RI品系构建近交系，并对与臭氧诱导的急性肺损伤存活相关的QTL Aliq1进行RIST。
