Wang C S, Liau Y E, Huang J C, Wu T D, Su C C, Lin C H
Graduate Institute of Agricultural Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Plant Cell Physiol. 1998 Dec;39(12):1307-14. doi: 10.1093/oxfordjournals.pcp.a029335.
This work characterizes a lily (Lilium longiflorum Thunb. cv. Snow Queen) anther (LLA) protein associated with desiccation. Peptide mapping analysis revealed that the abundant LLA-23 doublet contained similar polypeptides, having an isoelectric point of 6.1. Immunoblots of pollen protein from developing anther/pollen confirmed that the LLA-23 protein accumulated only at the later stage of pollen maturation and that the levels remained steady in mature and vital pollen. The accumulation of LLA-23 proteins was correlated with desiccation that naturally occurred in pollen. Subcellular fractionation of pollen proteins revealed that the protein was located in the cytoplasmic fraction. Premature drying of developing pollen confirmed that the concomitant accumulation of LLA-23 was associated with desiccation. Peptide sequence analysis demonstrates similarities between the lily LLA-23 and a family of water-deficit/ripening-induced proteins including LP3 of pine, DS2 of potato, and Asr of tomato and pummelo. In addition, the concomitant accumulation of LLA-23 can be experimentally manipulated by methyl jasmonate (Me-JA) and salicylic acid (SA) as well as by mannitol and methyl viologen. The LLA-23 represents a novel member of the water-deficit/ripening-induced proteins.
这项研究对一种与干燥相关的麝香百合（Lilium longiflorum Thunb. cv. Snow Queen）花药（LLA）蛋白进行了表征。肽图分析显示，丰富的LLA - 23双峰包含相似的多肽，其等电点为6.1。对发育中的花药/花粉的花粉蛋白进行免疫印迹分析证实，LLA - 23蛋白仅在花粉成熟后期积累，并且在成熟且有活力的花粉中水平保持稳定。LLA - 23蛋白的积累与花粉中自然发生的干燥相关。花粉蛋白的亚细胞分级分离显示该蛋白位于细胞质部分。发育中的花粉过早干燥证实LLA - 23的伴随积累与干燥有关。肽序列分析表明，麝香百合LLA - 23与一类水分亏缺/成熟诱导蛋白相似，包括松树的LP3、马铃薯的DS2以及番茄和柚子的Asr。此外，LLA - 23的伴随积累可以通过茉莉酸甲酯（Me - JA）、水杨酸（SA）以及甘露醇和甲基紫精进行实验调控。LLA - 23代表了水分亏缺/成熟诱导蛋白家族的一个新成员。
