Genome-Wide Identification and Expression Pattern Analysis of the Late Embryogenesis Abundant (LEA) Family in Foxtail Millet ( L.).

: Late embryogenesis abundant (LEA) proteins regulate stress responses and contribute significantly to plant stress tolerance. As a model species for stress resistance studies, foxtail millet () lacks comprehensive characterization of its gene family. This study aimed to comprehensively identify genes in foxtail millet and elucidate their functional roles and tissue-specific expression patterns. : Genome-wide identification of genes was conducted, followed by phylogenetic reconstruction, -acting element analysis of promoters, synteny analysis, and expression profiling. : Ninety-four genes were identified and classified into nine structurally distinct subfamilies, which are unevenly distributed across all nine chromosomes. Phylogenetic analysis showed closer clustering of genes with sorghum and rice orthologs than with genes. Synteny analysis indicated the gene family expansion through tandem and segmental duplication. Promoter -element analysis linked genes to plant growth regulation, stress responses, and hormone signaling. Transcriptome analysis revealed tissue-specific expression patterns among members, while RT-qPCR verified ABA-induced transcriptional regulation of genes. : This study identified 94 genes grouped into nine subfamilies with distinct spatial expression profiles. ABA treatment notably upregulated , , and in both shoots and roots.