BP2小鼠中的过敏性支气管收缩：5-羟色胺与乙酰胆碱之间的相互作用

Anaphylactic bronchoconstriction in BP2 mice: interactions between serotonin and acetylcholine.

Eum S Y, Norel X, Lefort J, Labat C, Vargaftig B B, Brink C

Unité de Pharmacologie Cellulaire, Unité Associée Institut Pasteur-INSERM 485, Institut Pasteur 25, Paris, France.

Br J Pharmacol. 1999 Jan;126(1):312-6. doi: 10.1038/sj.bjp.0702304.

Immunized BP2 mice developed an acute bronchoconstriction in vivo and airway muscle contraction in vitro in response to ovalbumin (OA) and these contractions were dose dependent. 2. Methysergide or atropine inhibited OA-induced bronchoconstriction in vivo and airway muscle contraction in vitro. 3. Neostigmine potentiated the OA-induced bronchoconstriction in vivo and airway muscle contraction in vitro of BP2 mice. This potentiation was markedly reduced by the administration of methysergide or atropine and when the two antagonists were administered together, the responses were completely inhibited. 4. Neostigmine also potentiated the serotonin (5-HT)- and acetylcholine (ACh)-induced bronchoconstriction and this potentiation was significantly reversed by atropine. 5. These results indicate that OA provokes a bronchoconstriction in immunized BP2 mice by stimulating the release of 5-HT, which in turn acts via the cholinergic mediator, ACh.

免疫后的BP2小鼠在体内出现急性支气管收缩，体外气道肌肉对卵清蛋白（OA）产生收缩反应，且这些收缩呈剂量依赖性。2. 甲基麦角新碱或阿托品可抑制体内OA诱导的支气管收缩以及体外气道肌肉收缩。3. 新斯的明增强了BP2小鼠体内OA诱导的支气管收缩和体外气道肌肉收缩。甲基麦角新碱或阿托品给药后，这种增强作用明显减弱，当两种拮抗剂联合给药时，反应被完全抑制。4. 新斯的明还增强了血清素（5-HT）和乙酰胆碱（ACh）诱导的支气管收缩，且阿托品可显著逆转这种增强作用。5. 这些结果表明，OA通过刺激5-HT释放，进而通过胆碱能介质ACh起作用，从而在免疫后的BP2小鼠中引发支气管收缩。

Anaphylactic bronchoconstriction in BP2 mice: interactions between serotonin and acetylcholine.